In vitro allosteric transcription factor-based biosensing

文献类型: 外文期刊

第一作者: Li, Shanshan

作者: Li, Shanshan;Li, Zilong;Xin, Zhenguo;Wang, Weishan;Tan, Gao-Yi;Tan, Gao-Yi;Xin, Zhenguo;Wang, Weishan

作者机构:

期刊名称:TRENDS IN BIOTECHNOLOGY ( 影响因子:17.3; 五年影响因子:18.1 )

ISSN: 0167-7799

年卷期: 2023 年 41 卷 8 期

页码:

收录情况: SCI

摘要: A biosensor is an analytical device that converts a biological response into a measurable output signal. Bacterial allosteric transcription factors (aTFs) have been utilized as a novel class of recognition elements for in vitro biosensing, which circumvents the limitations of aTF-based whole-cell biosensors (WCBs) and helps to meet the increasing requirement of small-molecule biosensors for diverse applications. In this review, we summarize the recent advances related to the configuration of aTF-based biosensors in vitro. Particularly, we evaluate the advantages of aTFs for in vitro biosensing and highlight their great potential for the establishment of robust and easy-to-implement biosensing strategies. We argue that key technical innovations and generalizable workflows will enhance the pipeline for facile construction of diverse aTF-based small-molecule biosensors.

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