Preparation of monoclonal antibodies recognizing pharmacologically active metabolites of metamizole based on rational hapten design and their application in the detection of animal-derived food

文献类型： 外文期刊

第一作者： Zhang, Linwei

作者： Zhang, Linwei;Wang, Jiacan;Wang, Yiting;Wen, Hao;Ding, Mingyue;Xiao, Jiaxu;Yang, Hongfei;Pan, Xiaoming;Han, Shiyun;Peng, Dapeng;Zhang, Linwei;Wang, Jiacan;Wang, Yiting;Wen, Hao;Ding, Mingyue;Xiao, Jiaxu;Yang, Hongfei;Pan, Xiaoming;Han, Shiyun;Peng, Dapeng;Peng, Dapeng;Peng, Dapeng

作者机构：

关键词： Metamizole; Monoclonal antibody; Hapten design; ic-ELISA

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）

ISSN： 0039-9140

年卷期： 2024 年 280 卷

页码：

收录情况： SCI

摘要： Metamizole (MET) is an antipyretic and analgesic drug, the illegal use of which can result in residues of MET metabolites in edible tissues of animals. In this study, a computational chemistry-assisted hapten screening strategy was used to screen for the optimal immunogenic hapten-A and the optimal coating antigen hapten-GOVA. A monoclonal antibody capable of recognizing two pharmacologically active metabolites of MET, 4-methylamidinoantipyrine (MAA) and 4-aminoantipyrine (AA), was prepared from the hapten-A. The antibody showed excellent specificity for MAA and AA and almost no cross-reactivity with the pharmacologically inactive metabolites 4-formamidinoantipyrine (FAA) and 4-acetamidinoantipyrine (AAA). An ic-ELISA was developed for the simultaneous detection of MAA and AA in animal-derived food, the limits of detection for MAA ranged from 0.93 to 1.18 mu g/kg, while those for AA ranged from 1.74 to 4.61 mu g/kg. The recovery rate fell within the range of 82 %-110 %, with a coefficient of variation less than 16.39 %.

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