Identification and Functional Analysis of the Ph-2 Gene Conferring Resistance to Late Blight (Phytophthora infestans) in Tomato
文献类型： 外文期刊
第一作者： Pan, Chunyang
作者： Pan, Chunyang;Li, Xin;Lu, Xiaoxiao;Hu, Junling;Zhang, Chen;Shi, Lianfeng;Zhu, Can;Guo, Yanmei;Wang, Xiaoxuan;Huang, Zejun;Du, Yongchen;Liu, Lei;Li, Junming
作者机构：
关键词：
tomato; late blight;
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2024 年 13 卷 24 期
页码：
收录情况： SCI
摘要： Late blight is a destructive disease affecting tomato production. The identification and characterization of resistance (R) genes are critical for the breeding of late blight-resistant cultivars. The incompletely dominant gene Ph-2 confers resistance against the race T1 of Phytophthora infestans in tomatoes. Herein, we identified Solyc10g085460 (RGA1) as a candidate gene for Ph-2 through the analysis of sequences and post-inoculation expression levels of genes located within the fine mapping interval. The RGA1 was subsequently validated to be a Ph-2 gene through targeted knockout and complementation analyses. It encodes a CC-NBS-LRR disease resistance protein, and transient expression assays conducted in the leaves of Nicotiana benthamiana indicate that Ph-2 is predominantly localized within the nucleus. In comparison to its susceptible allele (ph-2), the transient expression of Ph-2 can elicit hypersensitive responses (HR) in N. benthamiana, and subsequent investigations indicate that the structural integrity of the Ph-2 protein is likely a requirement for inducing HR in this species. Furthermore, ethylene and salicylic acid hormonal signaling pathways may mediate the transmission of the Ph-2 resistance signal, with PR1- and HR-related genes potentially involved in the Ph-2-mediated resistance. Our results could provide a theoretical foundation for the molecular breeding of tomato varieties resistant to late blight and offer valuable insights into elucidating the interaction mechanism between tomatoes and P. infestans.
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