Effects of berberine combined with Pennisetum sinese Roxb meal on growth, hepatic lipid metabolism, and intestinal health of grass carp, Ctenopharyngodon idella

文献类型： 外文期刊

第一作者： Tian, Jingjing

作者： Tian, Jingjing;Wang, Binbin;Ji, Mengmeng;Gong, Wangbao;Li, Hongyan;Xia, Yun;Zhang, Kai;Li, Zhifei;Xie, Wenping;Wang, Guangjun;Xie, Jun

作者机构：

关键词： Eco-friendly feed; Entero-hepatic health; Sustainable aquaculture; Chinese herbal medicine; High-density intensive aquaculture

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN： 2352-5134

年卷期： 2025 年 41 卷

页码：

收录情况： SCI

摘要： Intestinal inflammation and hepatic fat accumulation syndrome is a major issue in farmed fish. In this study, grass carp (20.3 +/- 0.1 g) were fed for 8 weeks with six feeds containing different Pennisetum sinese Roxb meal (PSRM) levels (0, 5, 10 %) and 0 or 0.1 % berberine (BBR). Growth remained unaffected by BBR and PSRM. PSRM reduced the CF, HI, and VSI, whereas BBR had no such effect or interaction. PSRM (not BBR) suppressed the activities of serum AST and ALT, as well as the contents of LDL - c, T - CHO, and TG (P < 0.05). However, BBR only interacted with the effects of PSRM on the AST and ALT activities (P < 0.01). BBR and PSRM decreased hepatic lipid droplet deposition and TG/T - CHO contents (P < 0.01), without interaction. Transcriptionally, both BBR and PSRM suppressed the lipogenic genes PPAR gamma, ACC, and SCD (P < 0.01) and upregulated the lipolytic genes PPAR alpha, ATGL, and APOE (P < 0.01), mostly without interaction. Intestinal immunohistochemistry revealed TNF - alpha below intestinal villi and IL - 6 on the upper side of villi and in lamina propria, both reduced in fish fed PSRM - and BBR - supplemented diets (P < 0.05). BBR and PSRM suppressed pro - inflammatory (TNF - alpha, IL - 6, IL - 8) and ER stress genes (GPR78, ATF6, IRE - 1 alpha, PERK), and promoted anti - inflammatory (TGF - beta 1, IL - 4/13 A), with interaction on TNF - alpha, IL - 8, GRP78, PERK, TGF - beta 1 (P < 0.05). BBR and PSRM triggered the FXR signaling pathway in liver and intestine, with BBR interacting with PSRM on most genes. Overall, BBR didn't disrupt PSRM-induced benefits on body morphology, had no superimposed effect on intestinal health but a synergistic effect on fat reduction.

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