Impact of quercetin on autophagy and apoptosis induced by a high concentration of CuSO4 in porcine ovarian granulosa cells

文献类型： 外文期刊

第一作者： Qi, Nannan

作者： Qi, Nannan;Wang, Binbin;Xing, Wenwen;Li, Mengxuan;Liu, Jiying;Qi, Nannan;Wang, Binbin;Xing, Wenwen;Li, Mengxuan;Liu, Jiying

作者机构：

关键词： Apoptosis; Autophagy; CuSO4; Porcine ovarian granulosa cells; Quercetin

期刊名称：DOMESTIC ANIMAL ENDOCRINOLOGY （ 影响因子：2.1； 五年影响因子：2.2 ）

ISSN： 0739-7240

年卷期： 2025 年 90 卷

页码：

收录情况： SCI

摘要： Copper is a vital micronutrient necessary for the maintenance of physiological functions. However, excessive amounts can lead to organ damage. Porcine ovarian granulosa cells are damaged by a high concentration of CuSO4, which can reduce the reproductive capacity of sows. Quercetin has shown remarkable efficacy in mitigating the harmful effects of heavy metals. Therefore, the aim of this study was to investigate the effects of a high concentration of CuSO4 on autophagy and apoptosis in porcine ovarian granulosa cells and to explore whether quercetin can counteract these toxic effect. Cell morphology, and the mRNA expression levels of autophagy-related genes (LC3-I, ATG5, ATG7, ATG12, Beclin1, mTOR, LC3-II and P62) were significantly changed upon treatment with 200 and 400 mu M CuSO4. Treatment with 200 mu M CuSO4 increased expression of P62 protein (P<0.05), promoted LC3-I to LC3-II conversion (P<0.05), and reduced PINK1 protein expression and the ATP content (P<0.05). In addition, expression of Caspase3 protein was increased and TUNEL staining indicated that the number of apoptotic cells was increased. However, co-treatment with 10 mu M quercetin significantly decreased expression of P62 and conversion of LC3-I to LC3-II. Furthermore, flow cytometric analysis revealed that addition of 10 mu M quercetin significantly reduced apoptosis induced by a high concentration of CuSO4. In summary, the results indicate that a high concentration of CuSO4 can trigger mitochondrial and autophagy dysfunction, activate mitochondrial apoptosis pathway, and exert cytotoxic effects. Quercetin can mitigate autophagy dysfunction, enhance autophagic processes, and alleviate apoptosis.

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