Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Sha Zhenxia

作者： Sha Zhenxia;Ren Guocheng;Wang Xianli;Wang Na;Chen Songlin;Ren Guocheng;Wang Xianli

作者机构：

关键词： embryonic cell line;half smooth tongue sole;karyotype;GFP;LCDV;CSEC

期刊名称：ACTA OCEANOLOGICA SINICA （ 影响因子：1.431； 五年影响因子：1.445 ）

ISSN： 0253-505X

年卷期： 2010 年 29 卷 2 期

页码：

收录情况： SCI

摘要： A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole (Cynoglossus semilaevis). CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS (fetal bovine serum), 2.5 ng/cm(3) bEGF (basic fibroblast growth factor), 1 ng/cm(3) LIF (leukemia inhibitory factor) and 50 mmol/dm(3) 2-ME (2-mecaptoethanol). The cells grew well in the temperature range of 24-30 degrees C and the optimal growth temperature was 24 degrees C. FBS and bFGE concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect (CPE) after infection with lymphosystis disease virus (LCDV) in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus.

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