A polysaccharide from the seed of Gleditsia japonica var. delavayi: Extraction, purification, characterization and functional properties

文献类型： 外文期刊

第一作者： Liu, Yun

作者： Liu, Yun;Shi, Zhijiao;Peng, Xiaowei;Deng, Jia;Zhao, Ping;Xu, Jinyu;Zhang, Xia;Kan, Huan

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关键词： Gleditsia japonica var. delavayi seed; Polysaccharides; Extraction; Characterization; Functional properties

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2024 年 191 卷

页码：

收录情况： SCI

摘要： Gleditsia japonica var. delavayi is widely distributed in Yunnan and Guizhou, China, and the perisperm of its seeds is frequently used as a traditional banquet sweet food with transparent color, smooth-tasting, low-fat, and high carbohydrate. Herein, a cellulase-assisted extraction method was applied using response surface methodology to optimize the extraction of polysaccharides from the seeds of G. japonica var. delavayi. The highest extraction yield (82.23 +/- 0.50%) was obtained under 5% cellulase, 176 mL/g liquid-to-material ratio, and enzymolysis parameters of 55 degrees C for 90 min at pH 4.5. The purified seed polysaccharide (PS) primarily contained galactomannan (weight average molecular weight, 42.74 kDa), which was linked by the monosaccharide residues 1,6-alpha-Galp, 1,4,6-alpha-D-Galp, 1,2,4,6-beta-D-Manp, and 1,3,6-alpha-D-Galp. The PS obtained has a single-crystal structure, is thermodynamically stable, and its molecules aggregate in water. Furthermore, PS possesses high cholate binding capacity and a substantial inhibitory effect on alpha-glucosidase and alpha-amylase. Taken together, the findings of this report offer a theoretical foundation for developing and utilizing G. japonica var. delavayi.

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