Rapid Russula senecis identification assays using loop-mediated isothermal amplification based on real-time fluorescence and visualization

文献类型： 外文期刊

第一作者： Wang, Nan

作者： Wang, Nan;Gao, Jie;Yu, Wenjie;Li, Hui;Zhang, Juan;Xie, Ruibin;Chen, Ailiang;Tian, Enjing

作者机构：

关键词： Poisonous mushroom; Species identification; Loop-mediated isothermal amplification; Russula senecis; Visualization

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.56； 五年影响因子：5.365 ）

ISSN： 0175-7598

年卷期： 2022 年 106 卷 3 期

页码：

收录情况： SCI

摘要： Russula senecis, a common poisonous mushroom, is widely distributed in China. Mushroom poisoning is becoming a major threat to human health and its rate is increasing worldwide. For the first time, we developed a set of loop-mediated isothermal amplification (LAMP) assays based on a real-time fluorescence and a visualization method to detect R. senecis, and the visual LAMP reaction system was optimized to further shorten the reaction time. Both real-time LAMP and visual LAMP could detect as low as 3.2 pg of genomic DNA. In addition, fried and digested mushrooms were used to validate the proposed LAMP method, and mushroom mixtures with as low as 1% of the target species could be successfully detected, indicating that the LAMP assays established in this study had good applicability and could be used for clinical sample detection and forensic identification. Furthermore, the LAMP assays were proven to be comparable to the real-time PCR method.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Deciphering Trachinotus ovatus resistance to Streptococcus agalactiae:GWAS :GWAS identification of key SNPs and candidate genes

    作者：Sun, Yi-Yao;Gao, Jie;Liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Zhu, Ke-Cheng;Xian, Lin;Zhang, Dian-Chang;Sun, Yi-Yao;Liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Zhu, Ke-Cheng;Xian, Lin;Zhang, Dian-Chang;Liu, Bao-Suo;Zhang, Nan;Zhu, Ke-Cheng;Zhang, Dian-Chang;Gao, Jie

    关键词：GWAS; Trachinotus ovatus; Streptococcus agalactiae; SNP

  • A ratiometric fluorescent probe for the determination of quinolone antibiotics in milk based on N and S co-doped carbon quantum dots

    作者：Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Han, Jiajun;Gong, Wenwen

    关键词：Carbon quantum dot; Ratiometric fluorescence probe; Moxifloxacin; Gatifloxacin; Ofloxacin; Milk

  • Identification, characterisation and expression analysis of peanut sugar invertase genes reveal their vital roles in response to abiotic stress

    作者：Mao, Tingting;Zhang, Yaru;Xue, Wenwen;Jin, Yu;Zhao, Hongfei;Wang, Yibo;Wang, Shengnan;Zhuo, Shengjie;Gao, Feifei;Su, Yanping;Yu, Chunyan;Guo, Xiaotong;Sheng, Yuting;Zhang, Juan;Zhang, Hongxia;Mao, Tingting;Zhang, Yaru;Jin, Yu;Zhao, Hongfei;Wang, Yibo;Wang, Shengnan;Zhuo, Shengjie;Gao, Feifei;Su, Yanping;Yu, Chunyan;Guo, Xiaotong;Sheng, Yuting;Zhang, Juan;Zhang, Hongxia;Zhang, Hongxia

    关键词：Peanut; Abiotic stress; Sucrose invertase; Genome-wide analysis; Gene expression

  • Phased genomics reveals hidden somatic mutations and provides insight into fruit development in sweet orange

    作者：Wang, Nan;Chen, Peng;Xu, Yuanyuan;Guo, Lingxia;Li, Xianxin;Wang, Nan;Yi, Hualin;Larkin, Robert M.;Deng, Xiuxin;Xu, Qiang;Wang, Nan;Zhou, Yongfeng;Chen, Peng;Xu, Yuanyuan;Guo, Lingxia;Li, Xianxin;Yi, Hualin;Larkin, Robert M.;Deng, Xiuxin;Xu, Qiang;Zhou, Yongfeng

    关键词：

  • Fungal diversity notes 1717-1817: taxonomic and phylogenetic contributions on genera and species of fungal taxa

    作者：Liu, Shi-Liang;Wang, Xue-Wei;Razaghi, Parisa;Raza, Mubashar;Cai, Lei;Jiang, Shuhua;Zhang, Chao;Zhou, Li-Wei;Wang, Xue-Wei;Li, Guo-Jie;Deng, Chun-Ying;Rossi, Walter;Leonardi, Marco;Liimatainen, Kare;Niskanen, Tuula;Kekki, Tapio;Smith, Matthew E.;Ammirati, Joe;Ammirati, Joe;Bojantchev, Dimitar;Abdel-Wahab, Mohamed A.;Zhang, Ming;Tian, Enjing;Bau, Tolgor;Lu, Yong-Zhong;Zhang, Jing-Yi;Ma, Jian;Zhang, Jing-Yi;Ma, Jian;Du, Tian-Ye;Peng, Xing-Can;Hyde, Kevin D.;Dutta, Arun Kumar;Acharya, Krishnendu;Saha, Rituparna;Tarafder, Entaj;Du, Tian-Ye;Du, Tian-Ye;Peng, Xing-Can;Hyde, Kevin D.;Xu, Jize;Kim, Ji Seon;Lim, Young Woon;Kim, Ji Seon;Lim, Young Woon;Gerlach, Alice;Zeng, Nian-Kai;Han, Yun-Xiao;Raza, Mubashar;Calabon, Mark S.;Jones, E. B. Gareth;Kumar, T. K. Arun;Krishnapriya, K.;Thomas, Anjitha;Kaliyaperumal, Malarvizhi;Kezo, Kezhocuyi;Gunaseelan, Sugantha;Singh, Sanjay Kumar;Singh, Paras Nath;Lagashetti, Ajay Chandrakant;Pawar, Kadambari Subhash;Rana, Shiwali;Zhang, Chao;Zhang, Huang;Qing, Yun;Peng, Xing-Can;Wen, Ting-Chi;Peng, Xing-Can;Wen, Ting-Chi;Ramirez, Natalia A.;Niveiro, Nicolas;Li, Mei-Xiang;Yang, Zhu L.;Wu, Gang;Li, Mei-Xiang;Yang, Zhu L.;Wu, Gang;Tennakoon, Danushka S.;Suwannarach, Nakarin;Kumla, Jaturong;Lumyong, Saisamorn;Tennakoon, Danushka S.;Suwannarach, Nakarin;Kumla, Jaturong;Lumyong, Saisamorn;Kuo, Chang-Hsin;da Silva, Tatiane M.;Bezerra, Jadson D. P.;Souza-Motta, Cristina M.;He, Gang;Ji, Xiao-Hong;Wannathes, Nopparat

    关键词：95 new taxa; Six new records; Ascomycota; Basidiomycota

  • Stimuli-responsive biodegradable silica nanoparticles: From native structure designs to biological applications

    作者：Qi, Qianhui;Wang, Wei;Shen, Qian;Geng, Jiaying;An, Weizhen;Wu, Qiong;Yu, Changmin;Shen, Qian;Geng, Jiaying;An, Weizhen;Wu, Qiong;Yu, Changmin;Qi, Qianhui;Yu, Changmin;Wang, Nan;Zhang, Yu;Li, Xue;Li, Lin

    关键词：Biodegradation; Silica nanoparticles; Stimuli -responsive; Multiple frameworks; Biological applications

  • Near-gapless and haplotype-resolved apple genomes provide insights into the genetic basis of rootstock-induced dwarfing

    作者：Li, Wei;Li, Hui;Sun, Haochen;Wang, Shiyao;Wang, Zijun;Li, Yuqi;Zhou, Bowen;Fan, Xingqiang;Xiong, Yao;Wang, Yi;Xu, Xuefeng;Han, Zhenhai;Chu, Chong;Zhang, Hengtao;Foster, Toshi M.;Lopez-Girona, Elena;Yu, Jiaxin;Li, Yi;Ma, Yue;Zhang, Ke;Zhang, Ke;Han, Yongming;Deng, Cecilia H.

    关键词：

微信小程序