A novel transcription factor SgTCP24 positively regulates mogroside V biosynthesis by activating SgSQE, SgCS, and SgCYP87D18 expression in Siraitia grosvenorii

文献类型： 外文期刊

第一作者： Mu, Detian

作者： Mu, Detian;Shao, Yingying;Chen, Wenqiang;Lin, Xiaodong;Zhou, Huimin;Lu, Ying;Tang, Qi;Mu, Detian;Shao, Yingying;Luo, Zuliang;Mo, Changming;Lu, Ying;Tang, Qi;Zhao, Huan;Ma, Xiaojun;Luo, Zuliang;Mo, Changming;Wilson, Iain W.;Pan, Limei;Huang, Huaxue;Qiu, Deyou

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关键词： Siraitia grosvenorii; genome-wide; mogroside V; SgTCP24; key enzyme genes

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 234 卷

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收录情况： SCI

摘要： Siraitia grosvenorii, a important economic crop is now used as a sweetener because its bioactive compound, mogroside V, has high sweetness with zero calories. However, mogroside V only accumulates in S. grosvenorii fruits, and its content is low. Enhancing the production of mogroside V has become a major commercial and scientific endeavor. TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors (TFs) have a key role in regulating secondary metabolite biosynthesis, however, their role in regulating mogroside V is unclear. In this study, twenty-four SgTCP genes were identified based on genome-wide analysis and classified into two subgroups according to phylogenetic analysis. Based on the expression levels in different fruit development stages, co-expression network showed that SgTCP24 had a strong positive correlation with SgSQE, SgCS, and SgCYP87D18. Through the subcellular localization, yeast one hybrid, dual-luciferase, and electrophoretic mobility shift assay it was shown that SgTCP24 can directly bind and activate the expression of these three genes. When SgTCP24 was over-expressed in S. grosvenorii fruits, the production of mogroside V increased. Conversely, the content of mogroside V decreased in lines subjected to virus induced silencing. Our results provide a major insight into the molecular mechanism of TCP TFs in regulating the mogroside V biosynthesis, and offers a new method for enhancing the content of mogroside V in plants.

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