文献类型： 外文期刊

第一作者： Gao, Yi

作者： Gao, Yi;Deng, Lan;Chen, Yuanyuan;Qin, Peiyou;Zhao, Yuanyuan;Zhao, Xiaoyan;Wang, Dan;Zhao, Shuang;Gao, Yi;Deng, Lan;Chen, Yuanyuan;Liu, Wei

作者机构：

关键词： aquaporin; constipation; Pleurotus citrinopileatus; polysaccharide; tight junction proteins

期刊名称：FRONTIERS IN NUTRITION （ 影响因子：5.1； 五年影响因子：5.4 ）

ISSN： 2296-861X

年卷期： 2025 年 12 卷

页码：

收录情况： SCI

摘要： Introduction Constipation is a prevalent gastrointestinal disorder demanding effective therapeutic strategies. This study investigated the therapeutic potential of Pleurotus citrinopileatus polysaccharide (PCP-g), a novel galactoglucan, against sucralfate-induced constipation murine model, focusing on intestinal motility, fecal parameters, aquaporin signaling, and gut microbiota modulation.Methods PCP-g was purified from P. citrinopileatus and its physicochemical properties were characterized. To evaluate the effects of PCP, the research utilized intestinal motility assays, fecal analysis, and in vitro fermentation. The role of Aquaporin 3 (AQP3) in constipation, especially regarding the PKA - phosphorylation mechanism, was investigated. The influence of PCP-g on PKA, phosphorylated PKA, AQP3, and tight junction proteins were examined at both the mRNA and protein levels.Results PCP-g was identified as a homogeneous galactoglucan with a molecular weight of 7.49 x 103 kDa, characterized by a backbone consisting of 1 -> 4-linked glucose (Glcp) and branches of mannose (Manp) and Glcp. The composition of PCP-g includes Glc, Gal, Man, L-Fuc, Rha, GlcA, and Ara, in a molar ratio of 1.00:0.16:0.13:0.01:0.006:0.005:0.006. The oral administration of PCP-g resulted in a significant reduction in constipation symptoms, as indicated by an increase in fecal water content, normalization of pellet formation, enhancement of total fecal mass, decreased latency to the first stool, and improved intestinal propulsion. Furthermore, PCP-g was found to elevate the production of short-chain fatty acids (SCFAs) while simultaneously reducing intestinal gas. Mechanistically, PCP-g suppressed the PKA-dependent phosphorylation of AQP3, leading to the downregulation of AQP3 overexpression and enhanced colonic epithelial permeability. Concurrently, PCP-g reduced the expression of tight junction proteins ZO-1 and Occludin, contributing to the increase in fecal water content.Discussion PCP-g effectively alleviates constipation by enhancing intestinal motility and fecal hydration. It modulates the PKA-AQP3 signaling pathway to improve colonic water permeability and positively influences the gut environment through the generation of SCFAs. These findings suggest that PCP-g may serve as a promising therapeutic candidate for the treatment of constipation, operating through aquaporin signaling and the regulation of the gut environment. The study advocates for further clinical trials and highlights the potential of edible mushroom polysaccharides in the management of constipation.

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