A conserved viral amphipathic helix governs the replication site-specific membrane association

文献类型： 外文期刊

第一作者： Sathanantham, Preethi

作者： Sathanantham, Preethi;Zhao, Wenhao;He, Guijuan;Murray, Austin;Wang, Xiaofeng;Zhao, Wenhao;Fenech, Emma;Schuldiner, Maya;Diaz, Arturo

作者机构：

期刊名称：PLOS PATHOGENS （ 影响因子：7.464； 五年影响因子：7.913 ）

ISSN： 1553-7366

年卷期： 2022 年 18 卷 9 期

页码：

收录情况： SCI

摘要： Positive-strand RNA viruses assemble their viral replication complexes (VRCs) on specific host organelle membranes, yet it is unclear how viral replication proteins recognize and what motifs or domains in viral replication proteins determine their destinations. We show here that an amphipathic helix, helix B in replication protein 1 a of brome mosaic virus (BMV), is necessary for la's localization to the nuclear endoplasmic reticulum (ER) membrane where BMV assembles its VRCs. Helix B is also sufficient to target soluble proteins to the nuclear ER membrane in yeast and plant cells. We further show that an equivalent helix in several plant- and human-infecting viruses of the Alsuviricetes class targets fluorescent proteins to the organelle membranes where they form their VRCs, including ER, vacuole, and Golgi membranes. Our work reveals a conserved helix that governs the localization of VRCs among a group of viruses and points to a possible target for developing broad-spectrum antiviral strategies.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序