VdERG2 was involved in ergosterol biosynthesis, nutritional differentiation and virulence of Verticillium dahliae

文献类型： 外文期刊

第一作者： Lv, Junyuan

作者： Lv, Junyuan;Zhang, Xiaojian;Zhao, Lihong;Feng, Zili;Wei, Feng;Zhou, Jinglong;Feng, Hongjie;Zhu, Heqin;Zhang, Yalin;Zhang, Zhigang;Zhao, Ruiyuan;Li, Caihong;Zhang, Tao;Liu, Shichao

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关键词： Verticillium dahliae; Ergosterol biosynthesis; Nutritional differentiation; Cell wall stress; Oxidative stress; Virulence

期刊名称：CURRENT GENETICS （ 影响因子：2.695； 五年影响因子：2.632 ）

ISSN： 0172-8083

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收录情况： SCI

摘要： The ergosterol biosynthesis pathway plays an important role in model pathogenic bacteria Saccharomyces cerevisiae, but little is known about the biosynthesis of ergosterol in the pathogenic fungus Verticillium dahliae. In this study, we identified the VdERG2 gene encoding sterol C-8 isomerase from V. dahliae and investigated its function in virulence by generating gene deletion mutants (Delta VdERG2) and complemented mutants (C-Delta VdERG2). Knockout of VdERG2 reduced ergosterol content. The conidial germination rate and conidial yield of Delta VdERG2 significantly decreased and abnormal conidia were produced. In spite of VdERG2 did not affect the utilization of carbon sources by V. dahliae, but the melanin production of Delta VdERG2 was decreased in cellulose and pectin were used as the sole carbon sources. Furthermore, the Delta VdERG2 mutants produced less microsclerotia and melanin with a significant decrease in the expression of microsclerotia and melanin-related genes VaflM, Vayg1, VDH1, VdLAC, VdSCD and VT4HR. In addition, mutants Delta VdERG2 were very sensitive to congo red (CR), sodium dodecyl sulfate (SDS) and hydrogen peroxide (H2O2) stresses, indicating that VdERG2 was involved in the cell wall and oxidative stress response. The absence of VdERG2 weakened the penetration ability of mycelium on cellophane and affected the growth of mycelium. Although Delta VdERG2 could infect cotton, its pathogenicity was significantly impaired. These phenotypic defects in Delta VdERG2 could be complemented by the reintroduction of a full-length VdERG2 gene. In summary, as a single conservative secretory protein, VdERG2 played a crucial role in ergosterol biosynthesis, nutritional differentiation and virulence in V. dahliae.

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