Sustainable utilization of feather waste to produce alkaline serine protease by genetically engineered Bacillus amyloliquefaciens

文献类型： 外文期刊

第一作者： Zhao, Ziyue

作者： Zhao, Ziyue;Li, Zhou;Guo, Ailing;Wei, Xuetuan;Zhao, Ziyue;Li, Zhou;Guo, Ailing;Wei, Xuetuan;Zhao, Ziyue;Li, Zhou;Guo, Ailing;Wei, Xuetuan;Wei, Xuetuan

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关键词： feather waste; biotransformation; alkaline serine protease; Bacillus amyloliquefaciens; efficient secretion expression

期刊名称：JOURNAL OF CLEANER PRODUCTION （ 影响因子：11.1； 五年影响因子：11.0 ）

ISSN： 0959-6526

年卷期： 2024 年 434 卷

页码：

收录情况： SCI

摘要： In this study, the efficient production of alkaline serine protease from engineered Bacillus amyloliquefaciens was achieved using feather meal as nitrogen sources. By screening of signal peptides library, a recombinant strain HZ12/SP2 with alkaline protease activity of 399.00 U/mL was constructed, which was 357% higher than the control strain HZ12/SP0. Furthermore, the alkaline protease activity was increased by 47%, up to 586.73 U/mL by deletion of the genes pssA, clsA, and sigF. Finally, the feather meal was used as nitrogen sources, and the strain Delta FCP/SP2 produced a maximum protease activity of 688.42 U/mL, which was 580% higher than the strain HZ12/SP0. In conclusion, an efficient alkaline serine protease-expression strain was constructed, which realized the valuable reuse of feather waste as nitrogen sources.

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