Molecular cloning, characterization, and functional analysis of the uncharacterized C11orf96 gene

文献类型： 外文期刊

第一作者： Yang, Hongzao

作者： Yang, Hongzao;Chen, Shaoyu;Yuan, Ligang;Yang, Hongzao;Zhu, Jie;Guo, Hongyuan;Tang, Aoxing;Zhang, Da;Liu, Guangqing

作者机构：

关键词： C11orf96; Felis catus; Gene cloning; Expression patterns; Biological function

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.792； 五年影响因子：3.008 ）

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年卷期： 2022 年 18 卷 1 期

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收录情况： SCI

摘要： Background The mammalian genome encodes millions of proteins. Although many proteins have been discovered and identified, a large part of proteins encoded by genes are yet to be discovered or fully characterized. In the present study, we successfully identified a host protein C11orf96 that was significantly upregulated after viral infection. Results First, we successfully cloned the coding sequence (CDS) region of the cat, human, and mouse C11orf96 gene. The CDS region of the C11orf96 gene is 372 bp long, encodes 124 amino acids, and is relatively conserved in different mammals. From bioinformatics analysis, we found that C11orf96 is rich in Ser and has multiple predicted phosphorylation sites. Moreover, protein interaction prediction analysis revealed that the protein is associated with several transmembrane family proteins and zinc finger proteins. Subsequently, we found that C11orf96 is strictly distributed in the cytoplasm. According to the tissue distribution characteristics, C11orf96 is distributed in all tissues and organs, with the highest expression levels in the kidney. These results indicate that C11orf96 may play a specific biological role in the kidney. Conclusions Summarizing, these data lay the foundation for studying the biological functions of C11orf96 and for exploring its role in viral replication.

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