RNAi mediated silencing of ecdysis-triggering hormone receptors (ETHRs) disrupts olfaction behaviours in Mythimna separata (Lepidotera: Noctuidae)

文献类型： 外文期刊

第一作者： Yang, Qi

作者： Yang, Qi;Li, Bo-Liao;Yang, Qi;Wu, Jun-Xiang;Wu, Yu-Ting;Xu, Xiang-Li;Li, Mei-Mei

作者机构：

关键词： Armyworm; MsepETHR-A; MsepETHR-B; ( Z )-11-hexadecenal; Volatiles

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 320 卷

页码：

收录情况： SCI

摘要： Ecdysis-triggering hormone receptors (ETHRs), a class of G protein-coupled receptors, play vital roles in insect ecdysis, reproduction, and courtship. Mythimna separata (armyworm) (Lepidotera: Noctuidae) is a devastating pest of major grain crops. In armyworm, MsepETHR-A and MsepETHR-B are consistently expressed in the adult antennae of both sexes. Fluorescence in situ hybridisation confirmed that both receptors were localised in cells underlying the sensilla trichodea on the antennae. RNA interference (RNAi)-mediated silencing of MsepETHR-A and MsepETHR-B downregulated their respective gene expressions. Functional studies revealed that females injected with dsETHR-B showed significantly reduced electroantennogram (EAG) responses to esters, aldehydes, and alcohols. Yet the reactions of females injected with dsETHR-A to 25 host-plant volatiles were not significantly different from the controls. Notably, males injected with dsETHR-A and dsETHR-B showed significantly reduced EAG responses to the sex pheromone component (Z)-11-hexadecenal (Z11-16: Ald) as well as to multiple hostplant volatiles. Behavioural assays in dual-choice arenas further demonstrated that males displayed significantly reduced olfactory attraction to Z11-16: Ald after silencing MsepETHR-A and MsepETHR-B, respectively. The results not only broaden the understanding of the olfactory mechanism of Lepidoptera but also provide a potential molecular target for developing pest management strategies against M. separata.

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