Combined transcriptome and metabolome analyses reveal the mechanisms of ultrasonication improvement of brown rice germination

文献类型： 外文期刊

第一作者： Zhang, Guangchen

作者： Zhang, Guangchen;Xu, Jiaxin;Sun, Xue;Huang, Lihua;Liu, He;Wang, Yiqiao;Huang, Shaosong;Sun, Jian;Liu, Youhong;Sun, Jian

作者机构：

关键词： Brown rice; Germination rate; Transcriptome sequencing; Metabolome analysis; Energy metabolism

期刊名称：ULTRASONICS SONOCHEMISTRY （ 影响因子：9.336； 五年影响因子：8.772 ）

ISSN： 1350-4177

年卷期： 2022 年 91 卷

页码：

收录情况： SCI

摘要： This study investigated the effects of ultrasonication treatment on the germination rate of brown rice. Brown rice grains were subjected to ultrasound (40 kHz/30 min) and then incubated for 36 h at 37.C to germinate the seeds. Ultrasonic treatment increased the germination rate of brown rice by up to similar to 28 % at 30 h. Transcriptomic and metabolomic analyses were performed to explore the mechanisms underlying the effect of ultrasonic treatment on the brown rice germination rate. Comparing the treated and control check samples, 867 differentially expressed genes (DEGs) were identified, including 638 upregulated and 229 downregulated), as well as 498 differentially accumulated metabolites (DAMs), including 422 up accumulated and 76 down accumulated. Multi-omics analysis revealed that the germination rate of brown rice was promoted by increased concentrations of low-molecular metabolites (carbohydrates and carbohydrate conjugates, fatty acids, amino acids, peptides, and analogues), and transcription factors (ARR-B, NAC, bHLH and AP2/EREBP families) as well as increased carbon metabolism. These findings provide new insights into the mechanisms of action of ultrasound in improving the brown rice germination rate and candidate DEGs and DAMs responsible for germination have been identified.

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