Editing of the MeSWEET10a promoter yields bacterial blight resistance in cassava cultivar SC8

文献类型： 外文期刊

第一作者： Wang, Yajie

作者： Wang, Yajie;Li, Ruimei;Liu, Jiao;Guo, Jianchun;Yao, Yuan;Geng, Mengting;Zhang, Tong;Lu, Xiaohua;Zhen, Xinghou;Che, Yannian;Chen, Yinhua;Pan, Ranran

作者机构：

关键词： cassava bacterial blight; cassava cultivar SC8; CRISPR/Cas9; MeSWEET10a promoter

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.6 ）

ISSN： 1464-6722

年卷期： 2024 年 25 卷 10 期

页码：

收录情况： SCI

摘要： Cassava starch is a widely used raw material for industrial production and food source for people. However, cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) results in severe yield losses and is the most destructive bacterial disease in all worldwide cassava-growing regions. Xam11 is a highly pathogenic subspecies from China that infects the Chinese local cassava South China No. 8 (SC8) cultivar with marked symptoms. This study showed that the transcription activator-like effector TALE20(Xam11) of Xam11 strain regulates the expression of disease-susceptibility gene MeSWEET10a by binding to the EBETALE20 region of the MeSWEET10a promoter in cassava cultivar SC8. CRISPR/Cas9-generated mutations of the EBETALE20 region resulted in a significant reduction in MeSWEET10a expression after infection by Xam11, correlating with reduced disease symptoms, smaller lesion sizes and decreased bacterial proliferation compared with the wild type. Importantly, the edited plants maintained normal growth, development and yield characteristics under greenhouse conditions. The results lay a research foundation for breeding resistant cassava cultivar SC8 to bacterial blight.

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