The shrimp NF-κB pathway is activated to mediate the antioxidant defense and apoptosis under ammonia-N stress

文献类型： 外文期刊

第一作者： Zhuo, Hongbiao

作者： Zhuo, Hongbiao;Lin, Lanting;Zhang, Yuan;Fu, Shuo;Li, Jinyan;Zhou, Xiaoxun;Wu, Guangbo;Guo, Chaoan;Liu, Jianyong;Zhuo, Hongbiao;Guo, Chaoan;Liu, Jianyong

作者机构：

关键词： Litopenaeus vannamei; PDTC; Ammonia-N stress; Antioxidant defense; Apoptosis

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.9； 五年影响因子：4.2 ）

ISSN： 1050-4648

年卷期： 2025 年 161 卷

页码：

收录情况： SCI

摘要： Ammonia-N stress from aquaculture system can interfere physiological function in shrimp leading to constant oxidative stress and apoptosis, but little is known concerning the upstream regulators. In this study, survival analysis and Western blot was perform to determine the optimal concentration and inhibitory effect on NF-kappa B pathway inhibitor (PDTC). Then the influence of 30 mg/kg PDTC on the ammonia-N tolerance, antioxidation, and apoptosis in L. vannamei exposed to 20 mg/L NH4Cl were evaluated. The results showed that PDTC promoted the gills tissue damage, hemolymph ammonia-N accumulation, and total hemocyte count (THC) decrease, thus resulting in a decreased survival rate in ammonia-exposed shrimp. Furthermore, PDTC exacerbated the oxidative damage by downregulating the expression of antioxidant-related genes. In addition, PDTC could enhance the Caspase-3 activity and apoptosis rate in gills by modulating the expression of genes associated with the mitochondrial apoptosis pathway. In primary cultured hemocytes of L. vannamei treated with 10 mM NH4Cl, PDTC treatment resulted in elevated ROS level and Caspase-3 activity, but also a decrease in mitochondrial membrane potential (MMP) and cell viability. These findings indicate that NF-kappa B activation acts as a critical upstream regulator of antioxidant defense and apoptosis in shrimp under ammonia-N stress.

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