Genetic Regulation of Chlorophyll Biosynthesis in Pepper Fruit: Roles of CaAPRR2 and CaGLK2

文献类型： 外文期刊

第一作者： Sun, Huagang

作者： Sun, Huagang;Sun, Huagang;Zhang, Yiyue;Zhang, Lingkui;Wang, Xiang;Zhang, Kang;Cheng, Feng;Chen, Shumin

作者机构：

关键词： pepper fruit color; chlorophyll; genetic mapping; bulked segregant analysis (BSA)

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 16 卷 2 期

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收录情况： SCI

摘要： Background: Pepper (Capsicum annuum L.) is a widely cultivated vegetable crop worldwide, with its rich fruit colors providing unique visual traits and economic value. This study investigated the genetic basis of the immature green fruit color by constructing a F2 segregating population derived from a cross between yellow fruit C20 and green fruit C62 parent lines. Methods: Bulked segregant analysis sequencing (BSA-seq) was performed to identify genomic regions associated with fruit color. Candidate genes were pinpointed through functional annotation and genetic variation analysis, supported by SNP markers, genotype analysis, and transcriptome profiling. Results: Two genomic regions associated with fruit color were identified on chromosomes 1 (14.55-20.85 Mb) and 10 (10.15-22.85 Mb), corresponding to previously reported loci pc1 and pc10.1. Two chlorophyll synthesis-related genes, CaAPRR2 and CaGLK2, were identified as candidate regulators of fruit color. Mutations in these genes include a premature stop codon in both CaGLK2 and CaAPRR2. The mutation of CaAPRR2 and CaGLK2 jointly regulate the yellow fruit trait in pepper, with CaGLK2 being the major gene and CaAPRR2 being the minor gene. Transcriptome analysis showed that the expression levels of the two genes increased during the green ripening stage of the parent fruits, with higher expression levels of CaGLK2. Conclusions: This study identifies CaGLK2 and CaAPRR2 as key regulators of immature green fruit color in pepper, with CaGLK2 playing a predominant role. These findings provide a theoretical foundation and data support for elucidating the molecular regulatory mechanisms of fruit color and advancing marker-assisted breeding in pepper.

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