Effects of phosphoglycerate kinase 1 and pyruvate kinase M2 on metabolism and physiochemical changes in postmortem muscle

文献类型： 外文期刊

第一作者： Huang, Caiyan

作者： Huang, Caiyan;Zhang, Dequan;Wang, Zhenyu;Li, Shaobo;Chen, Li;Huang, Caiyan;Blecker, Christophe;Zhao, Yingxin;Xiang, Can;Chen, Li

作者机构：

关键词： PGK1; PKM2; Inhibitors; Activators; Postmortem muscle; Glycolysis

期刊名称：FOOD CHEMISTRY-X （ 影响因子：6.1； 五年影响因子：6.4 ）

ISSN： 2590-1575

年卷期： 2024 年 21 卷

页码：

收录情况： SCI

摘要： The objective of this work was to investigate the influence of phosphoglycerate kinase -1 (PGK1) and pyruvate kinase -M2 (PKM2) activity on glycolysis, myofibrillar proteins, calpain system, and apoptosis pathways of postmortem muscle. The activity of PGK1 and PKM2 was regulated by their inhibitors and activators to construct the postmortem glycolysis vitro model and then incubated at 4 degree celsius for 24 h. The results showed that compared to PGK1 and PKM2 inhibitors groups, the addition of PGK1 and PKM2 activators could accelerate glycogen consumption, ATP and lactate production, while declining pH value. Moreover, the addition of PGK1 and PKM2 activators could increase desmin degradation, mu-calpain activity, and caspase-3 abundance. Interestingly, troponin-T degradation was significantly increased both in PKM2 inhibitor and activator groups. It was suggested that PGK1 and PKM2 might be used as robust indicators to regulate meat quality by affecting the glycolysis, myofibrillar proteins, mu-calpain and apoptosis pathways in postmortem muscle.

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