Unveiling the CoA mediated salicylate catabolic mechanism in Rhizobium sp. X9

文献类型： 外文期刊

第一作者： Zhou, Yidong

作者： Zhou, Yidong;Gao, Siyuan;Zhang, Mingliang;Jiang, Wankui;Ke, Zhijian;Qiu, Jiguo;Hong, Qing;Xu, Jianhong

作者机构：

关键词： biodegradation; CoA mediated hydroxylation; Rhizobium sp; X9; salicylate

期刊名称：MOLECULAR MICROBIOLOGY （ 影响因子：3.501； 五年影响因子：3.996 ）

ISSN： 0950-382X

年卷期： 2021 年 116 卷 3 期

页码：

收录情况： SCI

摘要： Salicylate is a typical aromatic compound widely distributed in nature. Microbial degradation of salicylate has been well studied and salicylate hydroxylases play essential roles in linking the peripheral and ring-cleavage catabolic pathways. The direct hydroxylation of salicylate catalyzed by salicylate-1-hydroxylase or salicylate-5-hydroxylase has been well studied. However, the CoA mediated salicylate 5-hydroxylation pathway has not been characterized in detail. Here, we elucidate the molecular mechanism of the reaction in the conversion of salicylate to gentisate in the carbaryl-degrading strain Rhizobium sp. X9. Three enzymes (salicylyl-CoA ligase CehG, salicylyl-CoA hydroxylase CehH and gentisyl-CoA thioesterase CehI) catalyzed the conversion of salicylate to gentisate via a route, including CoA thioester formation, hydroxylation and thioester hydrolysis. Further analysis indicated that genes cehGHI are also distributed in other bacteria from terrestrial environment and marine sediments. These genomic evidences highlight the role of this salicylate degradation pathway in the carbon cycle of soil organic compounds and marine sediments. Our findings of this three-step strategy enhanced the current understanding of CoA mediated degradation of salicylate.

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