In situ investigation of detoxification and metabolic effects of polyfluoroalkyl substances on metal-organic frameworks combined with cell-cultured microfluidics

文献类型： 外文期刊

第一作者： Xu, Ning

作者： Xu, Ning;Du, Qiuling;Dong, Shujun;Cheng, Jie;Wang, Peilong;Lin, Haifeng;Lin, Jin-Ming

作者机构：

期刊名称：LAB ON A CHIP （ 影响因子：6.1； 五年影响因子：6.9 ）

ISSN： 1473-0197

年卷期： 2023 年 23 卷 13 期

页码：

收录情况： SCI

摘要： Over 9000 types of per- and polyfluoroalkyl substances (PFASs) have been produced that exhibit environmental persistence, bioaccumulation and biotoxicity, and pose a potential hazard to human health. Although metal-organic frameworks (MOFs) are promising structure-based materials for adsorbing PFASs, the enormous structural diversity and variability of the pharmacologic action of PFASs present challenges to the development of structure-based adsorbents. To address this issue, we propose an in situ platform for the high-throughput identification of efficient MOF sorbents that can adsorb PFASs and their metabolism using a filter-chip-solid phase extraction-mass spectrometry (SPE-MS) system. As a proof of concept, we screened BUT-16 as an attractive material for in situ fluorotelomer alcohol (FTOH) adsorption. The results demonstrated that FTOH molecules were adsorbed around the surface of the large hexagonal pores of BUT-16 by forming multiple hydrogen bonding interactions with its Zr-6 clusters. The FTOH removal efficiency of the BUT16 filter was 100% over a period of 1 min. To determine the FTOH metabolism effects in different organs, HepG2 human hepatoma, HCT116 colon cancer, renal tubular HKC, and vascular endothelial HUVEC cells were cultured on a microfluidic chip, and SPE-MS was used to track a variety of cell metabolites in real time. Overall, the filter-Chip-SPE-MS system is a versatile and robust platform for the real-time monitoring of noxious pollutant detoxification, biotransformation, and metabolism, which facilitates pollutant antidote development and toxicology assay.

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