Chitosan-platinum nanozyme-mediated ratiometric fluorescent immunosensor with dual catalytic cascades for sensitive detection of fenitrothion

文献类型： 外文期刊

第一作者： Liu, Jie

作者： Liu, Jie;Deng, Hao;Jia, Bao-Zhu;Liu, Jie;Luo, Lin;Wang, Hong;Xu, Zhen-Lin;Yin, Qing-Chun;Wu, Min-Fu

作者机构：

关键词： Nanozyme; Catalytic cascades; Ratiometric fluorescence; Immunosensor

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）

ISSN： 0039-9140

年卷期： 2025 年 293 卷

页码：

收录情况： SCI

摘要： Organophosphorus pesticide residues pose significant threats to food safety and environmental health, and their monitoring methods demand sensitivity and anti-interference capability. In this study, we developed a novel ratiometric fluorescent immunosensor for fenitrothion (FN) detection by employing chitosan-modified platinum nanoparticles (Ch-Pt NPs) as catalytic nanozymes. Ch-Pt NPs exhibit oxidase-mimicking activity, enabling the oxidation of ascorbic acid (AA) and o-phenylenediamine (OPD) to produce dehydroascorbic acid (DHAA) and 2,3-diaminophenazine (DAP, Em = 568 nm), respectively. Then, DHAA further reacted with the remaining OPD to generate quinoxaline derivatives (DFQ, Em = 430 nm). These two products further form ratiometric fluorescent signals. Taking AA as a breakthrough point, the dual-catalytic cascade of natural enzymes and nanozymes was achieved by the combination with the alkaline phosphatase (ALP) -based enzyme-linked immunosorbent assay (ALP-ELISA). Compared with the conventional colorimetric ELISA, the proposed sensor demonstrated a nearly 20-fold enhancement in sensitivity (detection limit: 0.48 ng/mL) and achieved satisfactory recoveries of 80.0-108.3 % in spiked samples. The ratiometric fluorescent immunosensor demonstrates outstanding performance and holds great potential for extensive applications in the field of pesticide monitoring.

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