Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae)

文献类型： 外文期刊

第一作者： Liu, XZ

作者： Liu, XZ;Zhang, SC;Zhang, YZ;Xu, YJ

作者机构：

关键词： cryopreservation;sperm;spotted halibut;Verasper variegate

期刊名称：INDIAN JOURNAL OF MARINE SCIENCES （ 影响因子：0.496； 五年影响因子：0.605 ）

ISSN： 0379-5136

年卷期： 2006 年 35 卷 1 期

页码：

收录情况： SCI

摘要： The sperm of spotted halibut Verasper variegates was cryopreserved by a simple and convenient protocol. The cryoprotectants used were dimethyl sulfoxide (Me2SO), glycerol (Gly), methanol (MeOH), Me2SO + egg yolks, Gly + egg yolk and MeOH + egg yolk. The percentage of forward motile sperm in semen cryopreserved in the different extenders with Me2SO, Gly, MeOH, Me2SO + egg yolk, Gly + egg yolk and MeOH + egg yolk was 40.5 +/- 4.2%, 49.5 +/- 4.2%, 38.3 +/- 2.4%, 65.0 +/- 4.0%, 67.5 +/- 2.9% and 49.5 +/- 8.4%, respectively. The fertilization and hatching rates of cryopreserved sperm were from 59 +/- 2.4% to 70 +/- 3.0%, and from 11 +/- 3.0% to 18 +/- 2.6%, respectively. These tended to be highest when Gly + egg yolk or Me2SO + egg yolk were used as cryoprotectants. Under SEM, it could be seen that while the majority of the freeze-thawed sperm remained morphologically normal, some exhibited damaged or lost mitochondria, which possibly caused the decrease in motility and fertility of the freeze-thawed sperm.

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