Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization

文献类型： 外文期刊

第一作者： Ji, XS

作者： Ji, XS;Chen, SL;Tian, YS;Yu, GC;Sha, ZX;Xu, MY;Zhang, SC

作者机构：

关键词： cryopreservation;sperm;motility score;fertilization;sea perch;Lateolabrax japonicus

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2004 年 241 卷 1-4 期

页码：

收录情况： SCI

摘要： With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch (Lateolahrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw spermatozoa were examined. Post-thaw motility of frozen sperm obtained with extender modified plaice Ringer solution (MPRS) was higher than those achieved with extenders D-15 and modified Mounib's medium (MMM). With MPRS, the most effective cryoprotectant was determined to 10% dimethyl sulphoxide (DMSO). Post-thaw motility of frozen semen was not significantly reduced when the volume of diluted semen in the cryovial was increased from 0.5 to 1.0 ml (p>0.05). When the sperm/egg ratios varied from 320,000: 1 to 20,000: 1, fertilization rates of frozen semen cryopreserved for 3 days or 1 year in liquid nitrogen were not significantly different from that of fresh sperm (p>0.05). In fertilization trials of 230-ml eggs with frozen semen cryopreserved for 3 days in liquid nitrogen, 84.8% fertilization rate and 70.1% hatching rate were obtained, which was similar to control (81.0 +/- 2.3% and 87.2 +/- 3.1%) (p>0.05). Insemination of large egg batches (440-ml eggs) with frozen sperm cryopreserved for 1 year in liquid nitrogen resulted in high fertilization rates (83.5%) and hatching rates (90.0%) that resembled rates obtained with fresh (control) semen (p>0.05). Scanning and transmission electron microscopic observation indicated that while most of frozen-thawed sperm remained morphologically normal, some exhibited more or less damage, which probably caused the decrease in motility and fertility of the post-thawed sperm. (C) 2004 Elsevier B.V. All rights reserved.

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