Cloning, characterization and expression analysis of interleukin-10 from the zebrarish (Danio rerion)

文献类型： 外文期刊

第一作者： Zhang, DC

作者： Zhang, DC;Shao, YQ;Huang, YQ;Jiang, SG

作者机构：

关键词： cytokine;genomic analysis;interleukin;zebrafish

期刊名称：JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：2.021； 五年影响因子：2.1 ）

ISSN： 1225-8687

年卷期： 2005 年 38 卷 5 期

页码：

收录情况： SCI

摘要： Cytokines are proteins produced by many different cells of the immune system and play a significant role in initiating and regulating the inflammatory process. In this research, an important cytokine, interieukin-10 (IL-10) gene, has been identified and characterized from zebrafish (Danio rerio) genome database. Zebrafish IL-10 is located within a 2690 lip fragment and contains five exons and four introns, sharing the same organization with mammalian IL-10 genes. An open reading frame of 543 lip was found to encode a putative 180 amino acid protein with a signal peptide of 22 amino acids, which shares 29.7-80.9% homology with amino acid sequences of other known IL10. The signature motif of IL-10 is also conserved in zebrafish IL-10. The predicted transcript was finally confirmed by sequencing of cDNA clones. Multi-tissue reverse transcriptase PCR (RT-PCR) was performed to examine the tissue distribution and expression regulation of this gene in seven organs of normal and lipopolysaccharide (LPS) stimulation zebrafish. The results demonstrated that this gene was expressed slightly in normal kidney, gill and gut, no expression was detected in other four tissues. The expression was clearly upregulated after LPS stimulation. Using the ideal zebrafish model, further study of IL-10 characterization and function may provide insight on the understanding of the innate immune system.

分类号：

  • 相关文献

[1]Atypical Classical Swine Fever Infection Changes Interleukin Gene Expression in Pigs. Sun, Y. K.,Sun, Y. K.,Zhang, X. M.,Du, M.,Li, Y. X.,Pan, H. B.,Yan, Y. L.,Yang, Y. A..

[2]Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo. Gao, Yanni,Liu, Yongzhen,Guan, Xiaolu,Li, Xiaofei,Yun, Bingling,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Cui, Hongyu,Liu, Changjun,Zhang, Yanping,Wang, Xiaomei,Gao, Yulong,Wang, Xiaomei.

[3]Identification and genomic analysis of antifungal property of a tomato root endophyte Pseudomonas sp p21. Ma, Rongqin,Cheng, Zhiqiang,Lei, Shaonan,Li, Xin,Tian, Baoyu,Ma, Rongqin,Cheng, Zhiqiang,Lei, Shaonan,Li, Xin,Tian, Baoyu,Cao, Yi,Huang, Wei,Song, Yongkang.

[4]Genomic, evolutionary and expression profile analysis of Hsp70 superfamily in A and D genome of cotton (Gossypium spp.) under the challenge of Verticillium dahliae. Xiao, Songhua,Yu, Jingzhong,Yu, Deyue,Xiao, Songhua,Xu, Jianwen,Zhao, Jun,Liu, Jianguang,Wu, Qiaojuan. 2017

[5]Characterization of a virulent dog-originated rabies virus affecting more than twenty fallow deer (Dama dama) in Inner Mongolia, China. Zhu, Hongwei,Shao, Xiqun,Ba, Hengxing,Wang, Fengxue,Yang, Yong,Sun, Na,Ren, Jingqiang,Cheng, Shipeng,Wen, Yongjun,Chen, Xiaoyun,Wang, Hualei.

[6]Molecular cloning and characterization of Bombyx mori CREB gene. Song, Hongsheng,Sun, Yue,Zhang, Yang,Li, Muwang.

[7]Isolation of an anti-tumor polysaccharide from Auricularia polytricha (Jew's ear) and its effects on macrophage activation. Qing, Yuan,Yang, Zhirong,Zheng, Linyong,Zheng, Linyong,Yu, Mengyao,Xu, Xiaoyan,Qing, Yuan,Luo, Xia.

[8]The preparation of gypenosides liposomes and its effects on the peritoneal macrophages function in vitro. Yu, Yun,Bo, Ruonan,Huang, Yee,Hu, Yuanliang,Liu, Jiaguo,Wu, Yi,Tao, Yang,Wang, Deyun,Lu, Yu.

[9]Primary Babesia rodhaini infection followed by recovery confers protective immunity against B. rodhaini reinfection and Babesia microti challenge infection in mice. Wang, Guanbo,Efstratiou, Artemis,Moumouni, Paul Franck Adjou,Liu, Mingming,Jirapattharasate, Charoonluk,Guo, Huanping,Gao, Yang,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Zhou, Mo.

[10]Establishment of real-time TaqMan-fluorescence quantitative RT-PCR assay for detection and quantitation of ten kinds of porcine inflammation markers mRNA. Lin, Wencheng,Qiu, Zheng,Liu, Qinfang,Cui, Shangjin,Wang, Zhongtian.

[11]Construction of Eimeria tenella multi-epitope DNA vaccines and their protective efficacies against experimental infection. Song, Xiaokai,Xu, Lixin,Yan, Ruofeng,Huang, Xinmei,Li, Xiangrui,Huang, Xinmei.

[12]Effect of epimedium polysaccharide-propolis flavone immunopotentiator on immunosuppression induced by cyclophosphamide in chickens. Fan, Yunpeng,Wang, Deyun,Liu, Jiaguo,Zhao, Xiaojuan,Luong Nguyen,Hu, Yuanliang,Fan, Yunpeng,Song, Xiaoping,Zhang, Weimin,Lu, Yu.

[13]Expression of equine interleukin-18 by baculovirus expression system and its biologic activity. Wu, DL,Murakami, K,Liu, NH,Konishi, M,Muneta, Y,Inumaru, S,Kokuho, T,Sentsui, H. 2004

[14]Blumea balsamifera Oil for the Acceleration of Healing of Burn Injuries. Fan, Zuo-Wang,Pang, Yu-Xin,Wang, Kai,Yu, Fu-Lai,Wang, Dan,Ma, Qing-Song,Li, Xiao-Ting,Zou, Jin,Zhang, Wen-Qing,Wu, Li-Fen,Fan, Zuo-Wang,Yang, Quan,Ma, Qing-Song,Li, Xiao-Ting,Zou, Jin,Zhang, Wen-Qing,Wu, Li-Fen,Fan, Zuo-Wang,Pang, Yu-Xin,Wang, Kai,Yu, Fu-Lai,Wang, Dan,Ma, Qing-Song,Li, Xiao-Ting,Zou, Jin,Zhang, Wen-Qing,Wu, Li-Fen,Fan, Zuo-Wang,Pang, Yu-Xin,Wang, Kai,Yu, Fu-Lai,Wang, Dan,Ma, Qing-Song,Li, Xiao-Ting,Zou, Jin,Zhang, Wen-Qing,Wu, Li-Fen.

[15]Serum cytokine modulation after Staphylococcus hyicus infection in BALB/c mice. Li, Y.,Wang, L.,Zhang, L. Y.,Liu, Y. L.,Jiang, Z. Y.,Cai, R. J.,Wang, L.,Song, C. X.. 2015

[16]Macrophage immunomodulatory activity of polysaccharides isolated from Glycyrrhiza uralensis fish. Cheng, Anwei,Jin, Zhengyu,Xu, Xueming,Wan, Fachun,Wang, Jiaqi. 2008

[17]Eimeria tenella heat shock protein 70 enhances protection of recombinant microneme protein MIC2 subunit antigen vaccination against E. tenella challenge. Zhang, Lei,Ma, Liping,Liu, Renqiang,Zhang, Yunfei,Zhang, Shouping,Hu, Chunmei,Song, Meng,Wang, Ming,Cai, Jianping. 2012

[18]Infection of mouse bone marrow-derived immature dendritic cells with classical swine fever virus C-strain promotes cells maturation and lymphocyte proliferation. Zheng, Fu-Ying,Qiu, Chang-Qing,Jia, Huai-Jie,Chen, Guo-Hua,Zeng, Shuang,He, Xiao-Bing,Fang, Yong-Xiang,Lin, Guo-Zhen,Jing, Zhi-Zhong. 2013

[19]Therapeutic efficacy of Hypericum perforatum L. extract for mice infected with an influenza A virus. Pu Xiuying,Li Yan,Liang Jianping,Shang Ruofeng,Wang Xuehong,Liang Jianping,Zhou Liye. 2012

[20]Levamisole enhances immunity in ducklings vaccinated against Riemerella anatipestifer. Zhang, Yuewei,Zeng, Xiangfan,Wu, Wenxue,Chen, Huiling,Wang, Peng,Li, Jinxiang.

作者其他论文 更多>>

微信小程序