Integrated transcriptome and chromatin accessibility dynamics in developing walnut kernels reveal regulators of oil accumulation

文献类型： 外文期刊

第一作者： Zhang, Shulin

作者： Zhang, Shulin;Zhi, Zhaokun;Wang, Xiaofei;Jia, Wenqing;Zhou, Ruijin;Chen, Hongwei;Zhu, Gao-Pu;Zhao, Han;Zhang, Mengmeng;Zhu, Gao-Pu;Zhao, Han;Tian, Li;Chen, Xin

作者机构：

关键词： Juglans regia L; Transcriptome; Chromatin accessibility; Transcription factor

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 233 卷

页码：

收录情况： SCI

摘要： Walnuts are valued for their high lipid content, including polyunsaturated fatty acids. However, the molecular mechanisms driving oil biosynthesis during kernel development are not fully understood. To explore this, we constructed a comprehensive full-length transcriptome of walnut kernels across five developmental stages, identifying 221 candidate genes associated with oil biosynthesis, including 120 in fatty acid biosynthesis, 86 in triacylglycerol biosynthesis, and 15 in oil body formation. Integrating chromatin accessibility data with gene expression profiles, we found ten oil biosynthesis-related genes that showed coordinated changes in promoter accessibility and expression. Transcriptional regulatory network analysis further identified 23 hub transcription factors (TFs), including 14 putative negative regulators and 9 positive regulators of oil accumulation. Among these, JrERF93 as a member of AP2 family bound the promoter-accessible region of JrACC/CT alpha-5 and activated its expression in both yeast one-hybrid and dual-luciferase assay. Moreover, overexpression of JrERF93 in Arabidopsis thaliana significantly increased oil content and altered fatty acid composition in seeds. Collectively, these findings enhance our understanding the regulation of walnut oil biosynthesis and provide insights for strategies for genetic improvement.

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