Transcriptome and metabolome integrated analysis revealed the effects and potential mechanism of hydrogen peroxide on antioxidant system in postharvest broccoli

文献类型： 外文期刊

第一作者： Zhang, Yuxiao

作者： Zhang, Yuxiao;Chen, Ying;Guo, Yanyin;Sun, Yupeng;Wang, Zhengli;Wang, Yunqiao;Guan, Lingxing;Wang, Liang;Zhou, Qingxin

作者机构：

关键词： Ascorbic acid; Broccoli; Glucosinolate; Glutathione; Multi-omics; Reactive oxygen species

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：7.0； 五年影响因子：6.9 ）

ISSN： 0925-5214

年卷期： 2023 年 206 卷

页码：

收录情况： SCI

摘要： To investigate the effects and potential mechanism of exogenous H2O2 on antioxidant metabolism and yellowing in postharvest broccoli, the transcriptome and metabolome integrated analysis were performed. A total of 61 differentially expressed genes and 6 differentially accumulated metabolites identified among of H2O2 and diphenylene iodonium (DPI) treatments on 2 and 4 d. Exogenous H2O2 promoted the accumulation of endogenous H2O2 within 1 d by activating RBOHs expressions.H2O2 treatment up-regulated the expressions of SOD, CAT and peroxiredoxin (Prx) genes and APX, DHAR and GPX genes involved in ascorbic acid-glutathione (AsA-GSH) cycle, thereby promoting the scavenging of O-2 '(-) and H2O2 and alleviating the oxidative damage. Additionally, H2O2 treatment promoted the biosynthesis of AsA and GSH, and increased the content of AsA, GSH, oxidized glutathione, and.-glutamylcysteine.H2O2 treatment also promoted glucosinolate biosynthesis, the accumulation of 3-(6'-methylthio)hexylmalic acid, and the production of sulforaphane by up-regulating MYS expression, thereby enhancing the health-care function of postharvest broccoli. This study provided the new insights into regulatory mechanism of H2O2 on antioxidant system.

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