A novel raffinose biological pathway is observed by symbionts of cotton equivalent to Verticillium dahliae to improve salt tolerance genetically on cotton

文献类型： 外文期刊

第一作者： Cui, Ruifeng

作者： Cui, Ruifeng;Lu, Xuke;Chen, Xiugui;Malik, Waqar Afzal;Wang, Delong;Wang, Junjuan;Wang, Shuai;Guo, Lixue;Chen, Chao;Wang, Xiaoge;Wang, Xinlei;Dai, Maohua;Ye, Wuwei

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关键词： cotton; Cotton equivalent to VD Salt tolerance; raffinose; symbiont; Verticillium dahlia

期刊名称：JOURNAL OF AGRONOMY AND CROP SCIENCE （ 影响因子：3.473； 五年影响因子：4.395 ）

ISSN： 0931-2250

年卷期： 2021 年 207 卷 6 期

页码：

收录情况： SCI

摘要： Verticillium dahliae is a soil-borne, phytopathogenic fungus that causes Verticillium wilt in cotton. Salt tolerance of cotton plants suffering from Verticillium wilt was significantly improved, but the mechanism is unknown. In this study, we built a symbiont named Cotton equivalent to VD by immersing cotton plant roots into Verticillium dahliae liquid for four days. This resulted in a strong interaction between hyphae and the cotton roots. Under NaCl treatment for 24 h, the Cotton equivalent to VD displayed less leaf wilting and improved root growth compared with the control. Biochemical tests of SOD, POD and CAT showed significant overexpression in Cotton equivalent to VD compared with CK. RNA-seq data analysis of Cotton equivalent to VD indicated a novel 'galactose metabolism pathway' in the KEGG database, in which two key genes, GolS2 and RFS5, were significantly differentially expressed. Symbiotic metabolites inositol galactosyl could be transformed into raffinose to scavenge free radicals and participate in plant osmotic regulation to alleviate salt stress in Cotton equivalent to VD. Functional analysis indicated that silencing of the target gene GhRFS (RFS5) led to a decrease of raffinose content and salt tolerance as compared with CK. Cotton equivalent to VD can significantly improve cotton seedling salt tolerance through improving raffinose content, providing new insights into the salt tolerance mechanism and new salt-tolerant material in the future.

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