Construction of a density mutant collection in bitter gourd via new germplasms innovation and gene functional study

文献类型： 外文期刊

第一作者： Yu, Renbo

作者： Yu, Renbo;Niu, Yu;Yang, Kaili;Han, Xu;Liu, Zhaohua;Qi, Zhiqiang;Yang, Yan;Yu, Renbo;Niu, Yu;Yang, Kaili;Han, Xu;Qi, Zhiqiang;Yang, Yan;Yu, Renbo;Niu, Yu;Han, Xu;Liu, Zhaohua;Qi, Zhiqiang;Yang, Yan;Wang, Xiaoyi

作者机构：

关键词： bitter gourd; EMS; mutant collection; phenotype; germplasm

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Although a few studies have elucidated the creation of bitter gourd mutants, the suitable concentration and duration of ethyl methanesulfonate (EMS) mutagenesis have not been determined. In this study, mutant collection was conducted to create new germplasms and widen genetic diversity. By employing the seeds of the inbred line Y52 as the mutagenic material, EMS as the mutagen, and the suitable mutagenic conditions for bitter gourd seeds (EMS concentration 0.2%, mutagenic time 10 h), we mutated 10,000 seeds and acquired 3223 independent M-1 lines. For the randomly selected 1000 M-2 lines, 199 M-2 lines with visible phenotypes were found, and 167 M-2 lines were mutants of fruit shape, size, and tubercles. Furthermore, fourteen dwarf, eleven leaf color, five leaf shape, and eight meristem defect mutants were discovered in this mutant collection. In addition, three lines of 1253, 2284, and 3269 represented recessive mutants crossed with Y52. Furthermore, the yellow leaf lines of 2284 and 3269 were not mutated at the same gene locus. This study constructed a mutant collection through innovative new germplasms and provided valuable resources for bitter gourd breeding and functional gene research.

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