Dynamic patterns of gene expression and regulatory variation in the maize seed coat

文献类型： 外文期刊

第一作者： Li, Juan

作者： Li, Juan;Wang, Liangfa;Luo, Hongbing;Chen, Zehui;Li, Juan;Wang, Liangfa;Wan, Jiong;Dang, Kuntai;Meng, Shujun;Qiu, Xiaoqian;Zhao, Jiawen;Mu, Liqin;Ding, Dong;Tang, Jihua;Li, Juan;Wang, Liangfa;Lin, Yuan;Wang, Qiyue;Chen, Zehui;Tang, Jihua

作者机构：

关键词： Maize; Seed coat; Heterosis; RNA-seq

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.3； 五年影响因子：5.9 ）

ISSN： 1471-2229

年卷期： 2023 年 23 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundSeed size is an important factor contributing to maize yield, but its molecular mechanism remains unclear. The seed coat, which serves as one of the three components of the maize grain, determines seed size to a certain extent. The seed coat also shares the maternal genotype and is an ideal material for studying heterosis.ResultsIn this study, the self-pollinated seeds of the maize hybrid Yudan888 and its parental lines were continuously collected from 0 day after pollination (DAP) to 15 DAP for phenotyping, cytological observation and RNA-seq. The phenotypic data showed that 3 DAP and 8 DAP are the best time points to study maize seed coat heterosis. Cytological observations indicated that maize seed coat heterosis might be the result of the coordination between cell number and cell size. Furthermore, the RNA-seq results showed that the nonadditive genes changed significantly between 3 and 8 DAP. However, the number of genes expressed additively was not significantly different. Our findings suggest that seed coat heterosis in hybrid is the result of nonadditive expression caused by dynamic changes in genes at different time points during seed expansion and seed coat development. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment indicated that genes related to DNA replication, cell cycle regulation, circadian rhythms and metabolite accumulation contributed significantly to hybrid seed coat heterosis.ConclusionMaize seed coat phenotyping allowed us to infer that 3 DAP and 8 DAP are important time points in the study of seed coat heterosis. Our findings provide evidence for genes involved in DNA replication, cell cycle regulation, circadian rhythms and metabolite accumulation in hybrid with high or low parental expression as major contributors to hybrid seed coat heterosis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序