Multiplex-genome-editing based rapid directional improvement of complex traits in rice

文献类型： 外文期刊

第一作者： Wei, Yidong

作者： Wei, Yidong;Zhang, Haomin;Fan, Jiaxing;Cai, Qiuhua;Wang, Jinlan;Zhang, Min;Yan, Fengting;Jiang, Jiahuan;Xie, Hongguang;Luo, Xi;Wei, Linyan;Lin, Yuelong;He, Wei;Qu, Mengyu;Zhu, Yongsheng;Xie, Huaan;Zhang, Jianfu;Wei, Yidong;Zhang, Haomin;Fan, Jiaxing;Cai, Qiuhua;Wang, Jinlan;Zhang, Min;Yan, Fengting;Jiang, Jiahuan;Xie, Hongguang;Luo, Xi;Wei, Linyan;Lin, Yuelong;He, Wei;Qu, Mengyu;Zhu, Yongsheng;Xie, Huaan;Zhang, Jianfu;Fan, Jiaxing;Zhang, Zhixing;Zhang, Min;Yan, Fengting;Qu, Mengyu;Zhang, Xinxin

作者机构：

关键词： multiplex genome editing; rapid directional improvement; heading date; plant architecture; rice

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Although thousands of genes have been identified or cloned in rice (Oryza sativa) in the last two decades, the majority of them have only been separately characterized in specific varieties or single-gene modified backgrounds, thus limiting their practical application. We developed an optimized multiplex genome editing (MGE) toolbox that can efficiently assemble and stably express up to twelve sgRNA targets in a single plant expression vector. In this study, we established the MGE-based Rapid Directional Improvement (MRDI) strategy for directional improvement of complex agronomic traits in one small-scale rice transformation. This approach provides a rapid and practical procedure, encompassing sgRNA assembly, transgene-free screening and the creation of promising germplasm, by combining the precision of gene editing with phenotype-based field breeding. The MRDI strategy was used to generate the full diversity of twelve main agronomic genes in rice cultivar FXZ for the directional improvement of its growth duration and plant architecture. After applying the MRDI to FXZ, ideal plants with the desired traits of early heading date reduced plant height, and more effective panicles were generated without compromising yield, blast resistance and grain quality. Furthermore, the results of whole-genome sequencing (WGS), including the analysis of structural variations (SVs) and single nucleotide variations (SNVs) in the MGE plants, confirmed the high specificity and low frequency of unwanted mutations associated with this strategy. The MRDI breeding strategy would be a robust approach for exploring and applying crucial agronomic genes, as well as for generating novel elite germplasm in the future.

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