Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella

文献类型： 外文期刊

第一作者： Peng, Lu

作者： Peng, Lu;Zheng, Jun-Hao;Liu, Li-Li;Huang, Meng-Qi;Cao, Min-Hui;Cui, Jin-Dong;You, Min-Sheng;Zou, Ming-Min;Zou, Ming-Min;Peng, Lu;Zheng, Jun-Hao;Liu, Li-Li;Huang, Meng-Qi;Cao, Min-Hui;Cui, Jin-Dong;Vasseur, Liette;You, Min-Sheng;Peng, Lu;Zheng, Jun-Hao;Liu, Li-Li;Huang, Meng-Qi;Cao, Min-Hui;Cui, Jin-Dong;You, Min-Sheng;Peng, Lu;Zheng, Jun-Hao;Liu, Li-Li;Huang, Meng-Qi;Cao, Min-Hui;Cui, Jin-Dong;You, Min-Sheng;Vasseur, Liette

作者机构：

关键词： Plutella xylostella; Seminal fluid protein; Trypsin; Spermatogenesis; Genetic control

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 306 卷

页码：

收录情况： SCI

摘要： Insect seminal fluid proteins (SFPs) are primary factors affecting physiology and behavior in both sexes, making them valuable targets for pest control. However, SFPs have not been fully characterized in the Plutella xylostella, a global pest that attacks cruciferous crops. Here, 75 putative SFPs were identified in P. xylostella, compared to 10 orthologs in Drosophila melanogaster, 10 in Nilaparvata lugens, 5 in Apis mellifera, and 43 in Heliconius melpomene. Analyses of Ka/Ks suggested that SFPs had high evolution rates. Proteases (22/75, 29.3 %) accounted for the highest proportion of P. xylostella SFPs, including 16 trypsins. The phylogenetic analysis showed that most trypsins from P. xylostella and H. melpomene belonged to the same cluster. SFP04 (trypsin-1) was orthologous to the SFP ADJ58550.1 in H. melpomene. PxTry1 was specifically expressed in adult males and their accessory glands but was also detected in females after mating. A CRISPR/Cas9-induced PxTry1 homozygous mutant strain with a 22-base pair nucleotides insertion was generated. PxTry1 deletion resulted in swollen testes, smaller spermatophores, and abnormal sperm, thus reducing the P. xylostella egg production and hatching. These results clarify the role of insect SFPs in evolution and reproduction and identify a promising target for pest control based on genetic regulation.

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