Cysteine protease ervatamin-B-like-mediated spermatophore digestion and sperm release impair fertility of Plutella xylostella females

文献类型： 外文期刊

第一作者： Zheng, Junhao

作者： Zheng, Junhao;Zou, Mingmin;Qian, Yurui;Li, Ruonan;Bian, Huimin;Cao, Minhui;Huang, Mengqi;Cui, Jindong;Peng, Lu;Zheng, Junhao;Zou, Mingmin;Qian, Yurui;Li, Ruonan;Bian, Huimin;Cao, Minhui;Huang, Mengqi;Cui, Jindong;Peng, Lu;Zheng, Junhao;Zou, Mingmin;Qian, Yurui;Li, Ruonan;Bian, Huimin;Cao, Minhui;Huang, Mengqi;Cui, Jindong;Peng, Lu;Zheng, Junhao;Zou, Mingmin;Qian, Yurui;Li, Ruonan;Bian, Huimin;Cao, Minhui;Huang, Mengqi;Cui, Jindong;Peng, Lu;Zou, Mingmin

作者机构：

关键词： Plutellidae; bursa copulatrix; spermatheca; post-mating responses; reproductive regulation

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：4.6； 五年影响因子：4.4 ）

ISSN： 0171-8177

年卷期： 2025 年

页码：

收录情况： SCI

摘要： Mating induces post-mating responses in female insects, causing molecular, physiological, and behavioral changes. Spermatophore is a unique structure in females for seminal fluid storage in the bursa copulatrix, significant in sperm transfer and activation, and its digestion represents a crucial signal for female re-mating. Plutella xylostella, a lepidopteran pest that attacks cruciferous crops and wild plants, is a promiscuous insect that digests spermatophores rapidly. Molecular mechanisms underlying spermatophore digestion in P. xylostella are poorly understood. This study aimed to identify post-mating response proteins by using isobaric tags for relative and absolute quantification proteomics. A total of 843 proteins had significant differential expression between the reproductive glands of virgin and mated females, 720 of which were significantly upregulated after mating, including a cysteine protease family gene, cysteine protease ervata-min-B-like (PxCPEB), which is potentially related to spermatophore digestion. PxCPEB was specifically expressed in P. xylostella females, mostly in the female lower reproductive gland, but only in the bursa copulatrix and spermatheca, and PxCPEB transcription was significantly increased after mating. CRISPR/Cas9-induced PxCPEB knockout successfully produced a homozygous mutant strain with a 22-bp deletion. PxCPEB loss did not affect spermatogenesis but significantly prolonged the rate of spermatophore digestion, increased the apoptosis level of the bursa copulatrix, and inhibited sperm release and re-mating in females, significantly decreasing female fertility. PxCPEB, as a regulator of P. xylostella post-mating responses, is critical in spermatophore digestion, sperm release, re-mating, and female fertility and its potential as a promising target for genetic regulation-based pest control.

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