3D chromatin structures associated with ncRNA roX2 for hyperactivation and coactivation across the entire X chromosome

文献类型： 外文期刊

第一作者： Tian, Simon Zhongyuan

作者： Tian, Simon Zhongyuan;Yang, Yang;Ning, Duo;Jing, Kai;Xu, Yewen;Chen, Wei;Zheng, Meizhen;Tian, Simon Zhongyuan;Yang, Yang;Ning, Duo;Jing, Kai;Huang, Guangyu;Xu, Yewen;Yin, Pengfei;Chen, Gengzhan;Deng, Yuqing;Zhang, Shaohong;Zhang, Zhimin;Gao, Tong;Chen, Wei;Zheng, Meizhen;Fang, Ke;Li, Yiming;Huang, Haibo;Chen, Zhenxia;Li, Guoliang;Tian, Ruilin;Tian, Ruilin;Ruan, Yijun

作者机构：

期刊名称：SCIENCE ADVANCES （ 影响因子：11.7； 五年影响因子：13.7 ）

ISSN： 2375-2548

年卷期： 2024 年 10 卷 30 期

页码：

收录情况： SCI

摘要： The three-dimensional (3D) organization of chromatin within the nucleus is crucial for gene regulation. However, the 3D architectural features that coordinate the activation of an entire chromosome remain largely unknown. We introduce an omics method, RNA-associated chromatin DNA-DNA interactions, that integrates RNA polymerase II (RNAPII)-mediated regulome with stochastic optical reconstruction microscopy to investigate the landscape of noncoding RNA roX2-associated chromatin topology for gene equalization to achieve dosage compensation. Our findings reveal that roX2 anchors to the target gene transcription end sites (TESs) and spreads in a distinctive boot-shaped configuration, promoting a more open chromatin state for hyperactivation. Furthermore, roX2 arches TES to transcription start sites to enhance transcriptional loops, potentially facilitating RNAPII convoying and connecting proximal promoter-promoter transcriptional hubs for synergistic gene regulation. These TESs cluster as roX2 compartments, surrounded by inactive domains for coactivation of multiple genes within the roX2 territory. In addition, roX2 structures gradually form and scaffold for stepwise coactivation in dosage compensation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序