文献类型： 外文期刊
第一作者： Liao, Jingjing
作者： Liao, Jingjing;Xie, Lei;Cui, Shengrong;Luo, Zuliang;Ma, Xiaojun;Liu, Tingyao;Mo, Changming;Jia, Xunli;Huang, Xiyang
作者机构：
关键词： baicalein; multigene vector; Lycopersicon esculentum; plant chassis; biofortification; heterologous biosynthesis
期刊名称：MOLECULES （ 影响因子：4.927； 五年影响因子：5.11 ）
ISSN：
年卷期： 2022 年 27 卷 10 期
页码：
收录情况： SCI
摘要： Baicalein is a valuable flavonoid isolated from the medicinal plant Scutellaria baicalensis Georgi, which exhibits intensive biological activities, such as anticancer and antiviral activities. However, its production is limited in the root with low yield. In this study, In-Fusion and 2A peptide linker were developed to assemble SbCLL-7, SbCHI, SbCHS-2, SbFNSII-2 and SbCYP82D1.1 genes driven by the AtPD7, CaMV 35S and AtUBQ10 promoters with HSP, E9 and NOS terminators, and were used to engineer baicalein biosynthesis in transgenic tomato plants. The genetically modified tomato plants with this construct synthesized baicalein, ranging from 150 ng/g to 558 ng/g FW (fresh weight). Baicalein-fortified tomatoes have the potential to be health-promoting fresh vegetables and provide an alternative source of baicalein production, with great prospects for market application.
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