Heterologous mogrosides biosynthesis in cucumber and tomato by genetic manipulation

文献类型： 外文期刊

第一作者： Liao, Jingjing

作者： Liao, Jingjing;Xie, Lei;Qiao, Jing;Cui, Shengrong;Jia, Xunli;Luo, Zuliang;Ma, Xiaojun;Liu, Tingyao;Mo, Changming;Huang, Xiyang;Liao, Jingjing

作者机构：

期刊名称：COMMUNICATIONS BIOLOGY （ 影响因子：5.9； 五年影响因子：6.3 ）

ISSN：

年卷期： 2023 年 6 卷 1 期

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收录情况： SCI

摘要： Mogrosides are widely used as high-value natural zero-calorie sweeteners that exhibit an array of biological activities and allow for vegetable flavour breeding by modern molecular biotechnology. In this study, we developed an In-fusion based gene stacking strategy for transgene stacking and a multi-gene vector harbouring 6 mogrosides biosynthesis genes and transformed it into Cucumis sativus and Lycopersicon esculentum. Here we show that transgenic cucumber can produce mogroside V and siamenoside I at 587 ng/g FW and 113 ng/g FW, respectively, and cultivated transgenic tomato with mogroside III. This study provides a strategy for vegetable flavour improvement, paving the way for heterologous biosynthesis of mogrosides. Sweet vegetables are developed by introducing mogroside V synthase genes into cucumber and tomato using a multigene expression system based on In-fusion technology and self-cleaving 2A peptides.

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