An Enhanced Ratiometric Fluorescence Immunosensor for Rapid Detection of AFB1 Based on the Dual-Enzyme Synergistic Co-Catalytic Effect of Natural Enzymes and Nanozymes

文献类型： 外文期刊

第一作者： Jing, Yinnan

作者： Jing, Yinnan;Wang, Jiamin;Liu, Xuan;Li, Shurong;Yang, Qinkai;Wang, Mengnan;Fan, Xueyi;Ge, Guangsheng;Zheng, Kai;Wang, Jianlong;Wang, Yanru;Tang, Xiaoqian;Zhang, Qi

作者机构：

关键词： aflatoxin; anti-idiotypicnanobody; enzymesynergy; dual enzyme activity; ratiometric fluorescentsignal

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 14 期

页码：

收录情况： SCI

摘要： Aflatoxin B1 (AFB1) is considered a critical analyte in food safety testing due to its widespread contamination and high toxicity. In this study, a ratiometric fluorescent immunosensor with synergistic catalysis by natural enzymes and nanozymes (N-N-RF-immunosensor) was constructed for the rapid detection of AFB1. Copper oxide and zeolitic imidazolate framework composite nanomaterials (CuO/ZIF-8 NCM) with peroxidase and oxidase activities were prepared. A ratiometric fluorescence signal was generated through the synergistic cocatalytic effect of the CuO/ZIF-8 NCM and alkaline phosphatase, in response to the competition interaction between the anti-idiotypic nanobody-alkaline phosphatase fusion protein (Nb2-5-AP) and AFB1. The assay could detect aflatoxins within 140 min, exhibiting a half maximal inhibitory concentration (IC50) of 0.032 ng/mL, a limit of detection (LOD) of 2.4 pg/mL, and a linear range of 0.0024-1.043 ng/mL. Compared with the traditional enzyme-linked immunosorbent assay (ELISA), the sensitivity was enhanced 25-fold, while the detection parameter was shortened by 1/3. Furthermore, the immunosensor was successfully applied in the actual samples, with recoveries ranging from 81 to 127%. The N-N-RF-immunosensor shows great potential for rapid and sensitive detection of aflatoxins.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序