Integrated Analysis of Microarray, Small RNA, and Degradome Datasets Uncovers the Role of MicroRNAs in Temperature-Sensitive Genic Male Sterility in Wheat

文献类型： 外文期刊

第一作者： Liu, Yongjie

作者： Liu, Yongjie;Li, Dan;Zhang, Shengquan;Zhang, Liping;Gong, Jie;Li, Yanhong;Chen, Jiamin;Zhang, Fengting;Liao, Xiangzheng;Chen, Zhaobo;Wang, Yongbo;Pang, Binshuang;Ma, Jinxiu;Chen, Xianchao;Gao, Jiangang;Zhao, Changping;Gao, Shiqing;Liu, Yongjie;Zhang, Liping;Gong, Jie;Li, Yanhong;Chen, Jiamin;Pang, Binshuang;Zhao, Changping;Gao, Shiqing;Li, Dan

作者机构：

关键词： Triticum aestivum L; temperature-sensitive genic male sterility (TGMS); miRNA; cell plate; auxin; lipid metabolism; degradome

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 15 期

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收录情况： SCI

摘要： Temperature-sensitive genic male sterile (TGMS) line Beijing Sterility 366 (BS366) has been utilized in hybrid breeding for a long time, but the molecular mechanism underlying male sterility remains unclear. Expression arrays, small RNA, and degradome sequencing were used in this study to explore the potential role of miRNA in the cold-induced male sterility of BS366. Microspore observation showed defective cell plates in dyads and tetrads and shrunken microspores at the vacuolated stage. Differential regulation of Golgi vesicle transport, phragmoplast formation, sporopollenin biosynthesis, pollen exine formation, and lipid metabolism were observed between cold and control conditions. Pollen development was significantly represented in the 352 antagonistic miRNA-target pairs in the integrated analysis of miRNA and mRNA profiles. The specific cleavage of ARF17 and TIR1 by miR160 and miR393 were found in the cold-treated BS366 degradome, respectively. Thus, the cold-mediated miRNAs impaired cell plate formation through repression of Golgi vesicle transport and phragmoplast formation. The repressed expression of ARF17 and TIR1 impaired pollen exine formation. The results of this study will contribute to our understanding of the roles of miRNAs in male sterility in wheat.

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