Identification of B-cell epitopes on structural proteins VP1 and VP2 of Senecavirus A and development of a multi-epitope recombinant protein vaccine

文献类型： 外文期刊

第一作者： Zhang, Zhongwang

作者： Zhang, Zhongwang;Yao, Fei;Lv, Jianliang;Ding, Yaozhong;Liu, Xinsheng;Zhang, Liping;Ma, Zhongyuan;Zhou, Peng;Wang, Yonglu;Guo, Huichen;Pan, Li

作者机构：

关键词： Senecavirus A; B-cell epitope; Multi-epitope vaccine; Piglets; Immune protection

期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2023 年 582 卷

页码：

收录情况： SCI

摘要： Senecavirus A (SVA) is an important pathogenic cause of vesicular disease in pigs worldwide. In this study, we screened the B-cell epitopes of SVA using a bioinformatics approach combined with an overlapping synthetic polypeptide method. Four dominant B-cell epitopes (at amino acid (aa) positions: 7-26, 48-74, 92-109, and 129-144) from the VP1 protein and five dominant B-cell epitopes (aa: 38-57, 145-160, 154-172, 193-208, 249-284) from the VP2 protein were identified. Multi-epitope genes comprising the identified B-cell epitope domains were synthesized, prokaryotic expressed, and purified, and their immune protection efficacy was evaluated in piglets. Our results showed that the multi-epitope recombinant protein rP2 induced higher neutralizing antibodies and provided 80% protection against homologous SVA challenge. Thus, the B-cell epitope peptides identified in this study are potential candidates for SVA vaccine development, and rP2 may offer safety and efficacy in controlling infectious SVA.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序