Dual roles of R-loops in the formation and processing of programmed DNA double-strand breaks during meiosis

文献类型： 外文期刊

第一作者： Liu, Chao

作者： Liu, Chao;Wang, Liying;Wei, Huafang;Li, Wei;Xu, Wei;Yang, Zhuo;Li, Kuan;Sun, Qianwen;Hu, Jun;Chen, Jia-Yu;Liu, Chao;Chen, Yinghong;Zhang, Ruidan;Xiao, Sai;Liu, Wenwen;Li, Wei;Yang, Zhuo;Li, Kuan;Sun, Qianwen;Chen, Yinghong;Zhang, Ruidan;Xiao, Sai;Liu, Wenwen;Li, Wei;Xu, Wei

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关键词： Meiosis; Meiotic recombination; Meiotic DSB hotspots; R-loops; Transcription-replication head-on collisions

期刊名称：CELL AND BIOSCIENCE （ 影响因子：7.5； 五年影响因子：7.9 ）

ISSN：

年卷期： 2023 年 13 卷 1 期

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收录情况： SCI

摘要： BackgroundMeiotic recombination is initiated by Spo11-dependent programmed DNA double-strand breaks (DSBs) that are preferentially concentrated within genomic regions called hotspots; however, the factor(s) that specify the positions of meiotic DSB hotspots remain unclear.ResultsHere, we examined the frequency and distribution of R-loops, a type of functional chromatin structure comprising single-stranded DNA and a DNA:RNA hybrid, during budding yeast meiosis and found that the R-loops were changed dramatically throughout meiosis. We detected the formation of multiple de novo R-loops in the pachytene stage and found that these R-loops were associated with meiotic recombination during yeast meiosis. We show that transcription-replication head-on collisions could promote R-loop formation during meiotic DNA replication, and these R-loops are associated with Spo11. Furthermore, meiotic recombination hotspots can be eliminated by reversing the direction of transcription or replication, and reversing both of these directions can reconstitute the hotspots.ConclusionsOur study reveals that R-loops may play dual roles in meiotic recombination. In addition to participation in meiotic DSB processing, some meiotic DSB hotspots may be originated from the transcription-replication head-on collisions during meiotic DNA replication.

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