A xyloglucan endotransglucosylase/hydrolase gene, IbXTH16, increases cold tolerance in transgenic sweetpotato

文献类型： 外文期刊

第一作者： Yu, Tao

作者： Yu, Tao;Pan, Jiaquan;Liu, Sitong;Yang, Zitong;Liu, Zhenlei

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关键词： sweetpotato; cold tolerance; IbXTH16; BR; proline

期刊名称：FRONTIERS IN GENETICS （ 影响因子：2.8； 五年影响因子：3.3 ）

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年卷期： 2025 年 16 卷

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收录情况： SCI

摘要： Introduction Low temperature is a key environmental factor that threaten sweetpotato growth and development. In-depth studies on the gene functions underlying cold resistance are important for genetic engineering in sweetpotato.Methods The IbXTH16 gene was cloned using a homologous cloning approach. Its expression was detected in sweetpotato leaves subjected to low-temperature stress and brassinosteroid treatment. Subsequently, the IbXTH16 gene was introduced into sweetpotato variety Lizixiang to generate IbXTH16-overexpressing plants, thereby enabling the functional validation of the IbXTH16.Results and discussion The IbXTH16 gene was cloned from the cold-tolerant variety LHS21. Its 879 bp coding sequence encoded a 292 aa protein with a molecular weight of 32.983 kDa and a pI of 8.47. The 2039 bp genomic sequence of IbXTH16 contained two exons and one intron. The IbXTH16 protein was localized in the cell membrane. IbXTH16 was strongly induced by 4 degrees C and brassinosteroid. IbXTH16 positively regulates cold tolerance of sweetpotato by activating the BR and proline pathways.

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