Heat stress promotes haploid formation during CENH3-mediated genome elimination in Arabidopsis

文献类型： 外文期刊

第一作者： Jin, Chunlian

作者： Jin, Chunlian;Sun, Limin;Trinh, Hoang Khai;Danny, Geelen;Jin, Chunlian;Trinh, Hoang Khai

作者机构：

关键词： Haploid induction; Genome elimination; CENH3; Heat stress; Haploid induction ratio (HIR)

期刊名称：PLANT REPRODUCTION （ 影响因子：3.4； 五年影响因子：4.0 ）

ISSN： 2194-7953

年卷期： 2023 年 36 卷 2 期

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收录情况： SCI

摘要： Impaired activity of centromeric histone CENH3 causes inaccurate chromosome segregation and in crosses between the Arabidopsis recombinant CENH3 mutant GFP-tailswap and CENH3(G83E) with wild-type pollen it results in chromosome loss with the formation of haploids. This genome elimination in the zygote and embryo is not absolute as also aneuploid and diploid progeny is formed. Here, we report that a temporal and moderate heat stress during fertilization and early embryogenesis shifts the ratio in favour of haploid progeny in CENH3 mutant lines. Micronuclei formation, a proxy for genome elimination, was similar in control and heat-treated flowers, indicating that heat-induced seed abortion occurred at a late stage during the development of the seed. In the seeds derived from heat-treated crosses, the endosperm did not cellularize and many seeds aborted. Haploid seeds were formed, however, resulting in increased frequencies of haploids in CENH3-mediated genome elimination crosses performed under heat stress. Therefore, heat stress application is a selective force during genome elimination that promotes haploid formation and may be used to improve the development and efficacy of in vivo haploid induction systems.

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