Overexpression of sesame polyketide synthase A leads to abnormal pollen development in Arabidopsis

文献类型： 外文期刊

第一作者： Li, Tianyu

作者： Li, Tianyu;Yang, Yuanxiao;Liu, Hongyan;Dossou, Senouwa Segla Koffi;Zhou, Fang;Zhou, Ting;Zhao, Yingzhong

作者机构：

关键词： Sesame; Male sterility; Exine; Sporopollenin; SiPKSA

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.26； 五年影响因子：5.761 ）

ISSN： 1471-2229

年卷期： 2022 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Sesame is a great reservoir of bioactive constituents and unique antioxidant components. It is widely used for its nutritional and medicinal value. The expanding demand for sesame seeds is putting pressure on sesame breeders to develop high-yielding varieties. A hybrid breeding strategy based on male sterility is one of the most effective ways to increase the crop yield. To date, little is known about the genes and mechanism underlying sesame male fertility. Therefore, studies are being conducted to identify and functionally characterize key candidate genes involved in sesame pollen development. Polyketide synthases (PKSs) are critical enzymes involved in the biosynthesis of sporopollenin, the primary component of pollen exine. Their in planta functions are being investigated for applications in crop breeding. Results In this study, we cloned the sesame POLYKETIDE SYNTHASE A (SiPKSA) and examined its function in male sterility. SiPKSA was specifically expressed in sesame flower buds, and its expression was significantly higher in sterile sesame anthers than in fertile anthers during the tetrad and microspore development stages. Furthermore, overexpression of SiPKSA in Arabidopsis caused male sterility in transgenic plants. Ultrastructural observation showed that the pollen grains of SiPKSA-overexpressing plants contained few cytoplasmic inclusions and exhibited an abnormal pollen wall structure, with a thicker exine layer compared to the wild type. In agreement with this, the expression of a set of sporopollenin biosynthesis-related genes and the contents of their fatty acids and phenolics were significantly altered in anthers of SiPKSA-overexpressing plants compared with wild type during anther development. Conclusion These findings highlighted that overexpression of SiPKSA in Arabidopsis might cause male sterility through defective pollen wall formation. Moreover, they suggested that SiPKSA modulates vibrant pollen development via sporopollenin biosynthesis, and a defect in its regulation may induce male sterility. Therefore, genetic manipulation of SiPKSA might promote hybrid breeding in sesame and other crop species.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Antibacterial mechanism of vitamin C against Staphylococcus aureus, reduction of toxicity of metabolites and its application in pasteurized milk

    作者：Tian, Lei;Huang, Chunyang;Liu, Xing;Zhang, Yuxin;Zhang, Chunling;Wang, Xin;Zhou, Ting;Yu, Ying;Pan, Hu;Dai, Yanna;Pu, Jifeng;Fu, Chengyu;Zhang, Pengfei

    关键词：Vitamin C; Staphylococcus aureus; Antibacterial mechanism; Pasteurized milk

  • Authentication of edible herbal materials and food products using mass spectrometry based metabolites and inorganic constituents

    作者：Wadood, Syed Abdul;Nie, Jing;Li, Chunlin;Yuan, Yuwei;Wadood, Syed Abdul;Nie, Jing;Li, Chunlin;Yuan, Yuwei;Wadood, Syed Abdul;Nie, Jing;Li, Chunlin;Rogers, Karyne M.;Yuan, Yuwei;Xiao, Jianbo;Song, Yan;Liu, Hongyan;Wadood, Syed Abdul;Khan, Wahab Ali;Khan, Abbas;Rogers, Karyne M.

    关键词：Medicinal food homologous products; Geographical authentication; Adulteration; Metabolites; Stable isotopes; Food fraud

  • Chromosome-level genome assembly of a critically endangered species Leuciscus chuanchicus

    作者：Wang, Qi;Zhou, Qi;Liu, Hongyan;Li, Jiongtang;Jiang, Yanliang

    关键词：

  • Discrimination of different varieties of rice in Wuchang area based on E-nose and HS-SPME-GC-O-MS

    作者：Qian, Lili;Chen, Mingming;Song, Yan;Zuo, Feng;Qian, Lili;Qian, Lili;Zuo, Feng;Zhang, Tao;Liu, Xingquan;Zhou, Guoxin;Liu, Hongyan

    关键词：Rice; E-nose; Headspace solid-phase microextraction-gas; chromatography-olfactometry-mass spectrometry; Volatile compounds

  • Morus alba L. alleviates influenza viral pneumonia. Evidences of its mechanism of action

    作者：Liu, Yan;Wang, Junyi;Ye, Wenjing;Zhou, Ting;Zhu, Wenwen;Wu, Yueguo;Shi, Jing;Li, Yuanyuan;Jing, Junsong;Sun, Yi;Shui, Yiyang;Chen, Ranran;Wu, Lianhao;Xing, Lijuan;Huang, Rongrong;Zhang, Sheng;Shi, Jing;Li, Yuanyuan;Liu, Yan;You, Zhenqiang;Jing, Junsong;Sun, Yi;Shui, Yiyang;Chen, Ranran;Wu, Lianhao;Xing, Lijuan;Huang, Rongrong;You, Zhenqiang;Shi, Jing;Li, Yuanyuan;Liu, Yan;You, Zhenqiang

    关键词：Influenza viral pneumonia; C -type signaling receptor pathway; Dectin-1/Syk; Network pharmacology; Morus alba L.

  • The global spectrum of tree crown architecture

    作者：Jucker, Tommaso;Fischer, Fabian Jorg;Jackson, Toby D.;Chave, Jerome;Coomes, David A.;Caspersen, John;Ali, Arshad;Loubota Panzou, Grace Jopaul;Loubota Panzou, Grace Jopaul;Bocko, Yannick E.;Loumeto, Jean-Joel;Feldpausch, Ted R.;Falster, Daniel;Usoltsev, Vladimir A.;Adu-Bredu, Stephen;Alves, Luciana F.;Aminpour, Mohammad;Angoboy Ilondea, Bhely;Angoboy Ilondea, Bhely;Anten, Niels P. R.;Antin, Cecile;Barbier, Nicolas;Momo, Stephane T.;Pelissier, Raphael;Ploton, Pierre;Askari, Yousef;Ayyappan, Narayanan;Banin, Lindsay F.;Battles, John J.;O'Hara, Kevin L.;Beeckman, Hans;Bond-Lamberty, Ben;Bongers, Frans;Munoz, Rodrigo;Poorter, Lourens;Sterck, Frank J.;Bowers, Samuel;Godlee, John L.;Ryan, Casey;van Breugel, Michiel;van Breugel, Michiel;Hall, Jefferson S.;van Breugel, Michiel;Chantrain, Arthur;Doucet, Jean-Louis;Fayolle, Adeline;Chaudhary, Rajeev;Kunwar, Suwash;Dai, Jingyu;Liu, Hongyan;Dai, Jingyu;Liu, Hongyan;Dalponte, Michele;Dimobe, Kangbeni;Domec, Jean-Christophe;Domec, Jean-Christophe;Dupuy Rada, Juan Manuel;Hernandez-Stefanoni, Jose L.;Duursma, Remko A.;Enriquez, Moises;Meave, Jorge A.;Munoz, Rodrigo;van Ewijk, Karin Y.;Farfan-Rios, William;Fayolle, Adeline;Forni, Eric;Ferretti, Marco;Haeni, Matthias;Waldner, Peter;Zimmermann, Niklaus E.;Forrester, David I.;Gilani, Hammad;He, Jie-Kun;Jiang, Hai-Sheng;Hemp, Andreas;Higgins, Steven I.;Holdaway, Robert J.;Hussain, Kiramat;Hutley, Lindsay B.;Ichie, Tomoaki;Iida, Yoshiko;Joshi, Puspa Raj;Kaboli, Hasan;Kazempour Larsary, Maryam;Pourbabaei, Hassan;Kenzo, Tanaka;Kloeppel, Brian D.;Kohyama, Takashi S.;Kunwar, Suwash;Wang, Li-Qiu;Kuyah, Shem;Kvasnica, Jakub;Svatek, Martin;Lin, Siliang;Lines, Emily R.;Lorimer, Craig;Malhi, Yadvinder;Marshall, Peter L.;Mattsson, Eskil;Mattsson, Eskil;Matula, Radim;Mensah, Sylvanus;Mensah, Sylvanus;Mi, Xiangcheng;Wang, Weiwei;Momo, Stephane T.;Sonke, Bonaventure;Moncrieff, Glenn R.;Moncrieff, Glenn R.;Mora, Francisco;Munoz, Rodrigo;Nissanka, Sarath P.;Nur Hajar, Zamah Shari;Pearce, Steven;Sanger, Jennifer;Peri, Pablo L.;Pour, Mohsen Javanmiri;Ribeiro, Sabina C.;Sanaei, Anvar;Wirth, Christian;Schlund, Michael;Sellan, Giacomo;Sellan, Giacomo;Shenkin, Alexander;Takagi, Kentaro;Trugman, Anna T.;Vadeboncoeur, Matthew A.;Valipour, Ahmad;Valipour, Ahmad;Vanderwel, Mark C.;Vovides, Alejandra G.;Vovides, Alejandra G.;Wirth, Christian;Woods, Murray;Xiang, Wenhua;de Aquino Ximenes, Fabiano;Xu, Yaozhan;Xu, Yaozhan;Yamada, Toshihiro;Zavala, Miguel A.

    关键词：

  • F-box ubiquitin-ligating enzyme ZEITLUPE 1 inhibits the promotion of caffeic acid O-methyltransferase 1 in salt tolerance of apple by the multimeric E3 protein complex SCFZTL1-mediated ubiquitination modification and protein degradation

    作者：Shao, Liu;Chen, Shuqin;Liu, Jiaxiang;Yao, Xinjie;Zhou, Xiaolong;He, Yongqing;Li, Tianyu;Wang, Lin;Shao, Liu;Ju, Yutong;Wang, Lin;Yan, Tingting;Xiao, Kun;Mo, Weiping

    关键词：Post-translational modification; MdZTL1; MdCOMT1

微信小程序