Effectiveness of ruminal xylanase with an extra proline-rich C-terminus on lignocellulosic biomass degradation

文献类型： 外文期刊

第一作者： Dong, Ruyue

作者： Dong, Ruyue;Liao, Min;Qin, Xing;Wang, Xiaolu;Huang, Huoqing;Luo, Huiying;Yao, Bin;Bai, Yingguo;Tu, Tao;Liu, Xiaoqing;Penttinen, Leena;Hakulinen, Nina

作者机构：

关键词： GH10 family xylanase; C -terminus contributes; Catalytic efficiency; Biomass utilization

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2023 年 372 卷

页码：

收录情况： SCI

摘要： The efficient degradation of plant polysaccharides in agricultural waste requires xylanases with high catalytic activity. In this study, the C-terminal proline-rich GH10 xylanase XynA from sheep rumen was investigated using product analysis, structural characterization, truncated and site-directed mutagenesis, molecular dynamics simulation, and application evaluation, revealing that the proline-rich C-terminus contributes to the interaction at the substrate-binding pocket to reduce the binding free energy. Compared to the C-terminally truncated enzyme XynA-Tr, XynA has a more favorable conformation for proton transfer and affinity attack, facilitating the degradation of oligomeric and beechwood xylan without altering the hydrolysis pattern. Moreover, both the reduced sugar yield and weight loss of the pretreated wheat bran, corn cob, and corn stalk hydrolyzed by XynA for 12 h increased by more than 30 %. These findings are important to better understand the relationship between enzyme activities and their terminal regions and suggest candidate materials for lignocellulosic biomass utilization.

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