RNA interference targeting Ras GTPase gene Ran causes larval and adult lethality in Leptinotarsa decemlineata

文献类型： 外文期刊

第一作者： Shen, Chen-Hui

作者： Shen, Chen-Hui;Jin, Lin;Li, Guo-Qing;Fu, Kai-Yun;Fu, Kai-Yun;Guo, Wen-Chao

作者机构：

关键词： RNA interference; dietary introduction; bacteria-expressed dsRNA; LdRan

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.462； 五年影响因子：4.688 ）

ISSN： 1526-498X

年卷期： 2022 年 78 卷 9 期

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收录情况： SCI

摘要： BACKGROUND RNA interference (RNAi) is a breakthrough technology in pest control. It is highly efficient to Coleopteran pests such as the Colorado potato beetle Leptinotarsa decemlineata, a serious pest defoliator mainly attacking potatoes worldwide. The first step for effective pest control by RNAi is the development of effective and reliable target genes. RESULTS Our results revealed that continuous ingestion of dsLdRan for 3 days successfully silenced the target gene, inhibited larval growth and killed 100% L. decemlineata larvae. When the bioassay began at the second-, third/fourth-instar larval stages, the larval lethality mainly occurred at the fourth larval instar and prepupal stages, respectively. Importantly, consumption of dsLdRan for 3 days by the newly-emerged males and females effectively knocked down the target transcript, reduced fresh weights and caused 100% of lethality within a week. The LdRan females possessed underdeveloped ovaries. CONCLUSION Considering that the larvae, adults and eggs are simultaneously sited on the potato plants, bacterially-expressed dsLdRan is a potential RNAi-based strategy for managing L. decemlineata in the potato field.

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