Isolation, Identification and Characteristics of Aeromonas caviae from Diseased Largemouth Bass (Micropterus salmoides)

文献类型： 外文期刊

第一作者： Xue, Mingyang

作者： Xue, Mingyang;Xiao, Zidong;Li, Yiqun;Jiang, Nan;Liu, Wenzhi;Meng, Yan;Fan, Yuding;Zeng, Lingbing;Zhou, Yong;Xiao, Zidong

作者机构：

关键词： Micropterus salmoides; Aeromonas caviae; virulence genes; antibiotic susceptibility; histopathology

期刊名称：FISHES （ 影响因子：3.17； 五年影响因子：3.098 ）

ISSN：

年卷期： 2022 年 7 卷 3 期

页码：

收录情况： SCI

摘要： The largemouth bass (Micropterus salmoides) is one of the most economically valuable fish species in China. In this study, a bacterial pathogen was isolated from the internal organs of diseased M. salmoides, and the strain was named WH21406. This isolate was identified as Aeromonas caviae on the basis of its morphology, biochemical features and 16S rDNA phylogenetic analysis. Four virulence genes related to pathogenicity, namely, flagella (fla), elastase (ela), haemolysin (hly) and aerolysin (aer), were detected in this isolate. The median lethal dosage (LD50) of A. caviae WH21406 for M. salmoides was calculated to be 3.46 x 10(5) CFU mL(-1). The histopathological analysis showed obvious tissue damage in the gill, liver, kidney, spleen and gut of the diseased fish. The antibiotic susceptibility test demonstrated that strain WH21406 was highly sensitive to enrofloxacin, norfloxacin, streptomycin and amikacin. The results of this study provide a foundation for the diagnosis, prevention and treatment of A. caviae infection in M. salmoides.

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