Camellia sinensis WIP domain protein 3 (CsWIP3), a C2H2 zinc finger protein, mediates lignin content and regulates plant growth in tea plants

文献类型： 外文期刊

第一作者： Zhao, Zhen

作者： Zhao, Zhen;Wang, Xiaoxuan;Han, Rui;Zhao, Yuxin;Liu, Shujing;Zhuang, Jing;Wang, Yuhua;Chen, Xuan;Li, Xinghui;Liu, Benying

作者机构：

关键词： Camellia sinensis; CsWIP3; Lignin; Stunted growth

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 307 卷

页码：

收录情况： SCI

摘要： The WIP proteins are essential for plant development, but their functions in tea plants (Camellia sinensis) remain poorly understood. In this study, six WIP members were identified in the tea plants and conducted a systematic analysis of their structure characteristics, expression patterns, promoter cis-acting elements, and functional roles. Sequence alignment and phylogenetic analysis revealed that the CsWIP family contains members with characteristic C2H2 zinc finger domains. Expression analysis across different tissues revealed a constitutive expression pattern. Promoter cis-acting element analysis identified several key regulatory elements associated with growth, development, and stress responses, highlighting the potential regulatory roles of CsWIP genes. Subcellular localization studies showed that CsWIP proteins primarily localize in the nucleus. Overexpression of CsWIP3 in Arabidopsis thaliana led to stunted growth, reduced leaf size, and increased lignin content, indicating its role in plant growth and lignification, with its function also validated in Solanum lycopersicum. Additionally, yeast two- hybrid assays identified interactions between CsWIP3 and CsTTG, CsAim32, and CsDUF1005, all of which are involved in regulating plant development, flower formation, and lignin biosynthesis. This study provides new insights into the functions of the CsWIP gene family in tea plants, revealing their functional diversity and potential applications in enhancing growth and development in tea plants.

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