文献类型： 外文期刊

第一作者： Yao, Qianqian

作者： Yao, Qianqian;Gao, Yanan;Fan, Linlin;Wang, Jiaqi;Zheng, Nan;Yao, Qianqian;Gao, Yanan;Fan, Linlin;Wang, Jiaqi;Zheng, Nan;Yao, Qianqian

作者机构：

关键词： 2 '-fucosyllactose (2 '-FL); liver oxygen stress; gut microbiota; glycometabolism; lipid metabolism; Akkermansia muciniphila; Paraprevotella spp.

期刊名称：NUTRIENTS （ 影响因子：6.706； 五年影响因子：7.185 ）

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年卷期： 2022 年 14 卷 19 期

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收录情况： SCI

摘要： Liver oxygen stress is one of the main extraintestinal manifestations of colitis and 5% of cases develop into a further liver injury and metabolic disease. 2'-fucosyllactose (2'-FL), a main member of human milk oligosaccharides (HMOs), has been found to exert efficient impacts on remitting colitis. However, whether 2'-FL exerts the function to alleviate colitis-induced liver injury and how 2'-FL influences the metabolism via regulating gut microbiota remain unknown. Herein, in our study, liver oxygen stress was measured by measuring liver weight and oxygen-stress-related indicators. Then, 16S full-length sequencing analysis and non-target metabolome in feces were performed to evaluate the overall responses of metabolites and intestinal bacteria after being treated with 2'-FL (400 mg/kg b.w.) in colitis mice. The results showed that, compared with the control group, the liver weight of colitis mice was significantly decreased by 18.30% (p < 0.05). After 2'-FL treatment, the liver weight was significantly increased by 12.65% compared with colitis mice (p < 0.05). Meanwhile, they exhibited higher levels of oxidation in liver tissue with decreasing total antioxidant capacity (T-AOC) (decreased by 17.15%) and glutathione (GSH) levels (dropped by 22.68%) and an increasing malondialdehyde (MDA) level (increased by 36.24%), and 2'-FL treatment could reverse those tendencies. Full-length 16S rRNA sequencing revealed that there were 39 species/genera differentially enriched in the control, dextran sulphate sodium (DSS), and DSS + 2'-FL groups. After treatment with 2'-FL, the intestinal metabolic patterns, especially glycometabolism and the lipid-metabolism-related process, in DSS mice were strikingly altered with 33 metabolites significantly down-regulated and 26 metabolites up-regulated. Further analysis found DSS induced a 40.01%, 41.12%, 43.81%, and 39.86% decline in acetic acid, propionic acid, butyric acid, and total short chain fatty acids (SCFAs) in colitis mice (all p < 0.05), respectively, while these were up-regulated to different degrees in the DSS + 2'-FL group. By co-analyzing the data of gut microbiota and metabolites, glycometabolism and lipid-metabolism-associated metabolites exhibited strong positive/negative relationships with Akkermansia_muciniphila (all p < 0.01) and Paraprevotella spp. (all p < 0.01), suggesting that the two species might play crucial roles in the process of 2'-FL alleviating colitis-induced liver oxygen stress. In conclusion, in the gut-liver-microbiotas axis, 2'-FL mediated in glucose and lipid-related metabolism and alleviated liver oxygen stress via regulating gut microbiota in the DSS-induced colitis model. The above results provide a new perspective to understand the probiotic function of 2'-FL.

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