UNC93B1 attenuates the cGAS-STING signaling pathway by targeting STING for autophagy-lysosome degradation

文献类型： 外文期刊

第一作者： Zhu, Huifang

作者： Zhu, Huifang;Zhang, Rongzhao;Yi, Li;Zheng, Chunfu;Tang, Yan-Dong;Zheng, Chunfu

作者机构：

关键词： cellular signal transduction; disease control; herpes simplex virus; immune responses; innate immunity; interferons; research and analysis methods; virus classification

期刊名称：JOURNAL OF MEDICAL VIROLOGY （ 影响因子：20.693； 五年影响因子：12.203 ）

ISSN： 0146-6615

年卷期： 2022 年 94 卷 9 期

页码：

收录情况： SCI

摘要： Stimulator of interferon genes (STING) is a pivotal innate immune adaptor, and its functions during DNA virus infections have been extensively documented. However, its homeostatic regulation is not well understood. Our study demonstrates that Unc-93 homolog B1 (UNC93B1) is a crucial checker for STING to prevent hyperactivation. Ectopic expression of UNC93B1 attenuates IFN-beta promoter activity and the transcriptions of IFN-beta, ISG54, and ISG56 genes. Moreover, UNC93B1 also blocks the IRF3 nuclear translocation induced by ectopic expression of both cyclic GMP-AMP synthase (cGAS) and STING and reduces the stability of STING by facilitating its autophagy-lysosome degradation, which can be reversed by lysosome inhibitors. Mechanistically, UNC93B1 interacts with STING and suppresses STING-activated downstream signaling by delivering STING to the lysosomes for degradation, depending on its trafficking capability. UNC93B1 knockout in human embryonic kidney 293T cells facilitates IFN-beta promoter activity, IFN-beta, ISG54, and ISG56 transcriptions IRF3 nuclear translocation induced by ectopic expression of cGAS and STING. Infected with herpes simplex virus-1 (HSV-1), UNC93B1 knockdown BJ cells or primary peritoneal macrophages from Unc93b1-deficient (Unc93b1(-/-)) mice show enhanced IFN-beta, ISG54, and ISG56 transcriptions, TBK1 phosphorylation, and reduced STING degradation and viral replication. In addition, Unc93b1(-/-) mice exhibit higher IFN-beta, ISG54, and ISG56 transcriptions and lower mortality upon HSV-1 infection in vivo. Collectively, these findings demonstrate that UNC93B1 attenuates the cGAS-STING signaling pathway by targeting STING for autophagy-lysosome degradation and provide novel insights into the function of UNC93B1 in antiviral innate immunity.

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